Pedersen B K, Tvede N, Hansen F R, Andersen V, Bendix T, Bendixen G, Bendtzen K, Galbo H, Haahr P M, Klarlund K
Department of Medicine TTA, Rigshospitalet, Copenhagen, Denmark.
Scand J Immunol. 1988 Jun;27(6):673-8. doi: 10.1111/j.1365-3083.1988.tb02400.x.
The present study was designed to examine the effect of physical exercise on human natural killer (NK) cells. Six healthy volunteers underwent two different acute physical exercise tests with an interval of at least 1 week: (1) 60 min bicycle exercise at 80% of maximal oxygen uptake (VO2max) and (2) 60 min back-muscle training at up to 29% of VO2max; blood samples were collected before and during the last few minutes of exercise, as well as 2 h and 24 h afterwards. The NK cell activity (lysis/fixed number of mononuclear cells) increased during bicycle exercise, dropped to a minimum 2 h later and returned to pre-exercise levels within 24 h. Back-muscle exercise did not significantly influence NK cell activity. Plasma levels of adrenaline, noradrenaline, and cortisol were elevated during bicycling, but not during back-muscle exercise, indicating that exercise intensity is a determinant of NK cell activity. During bicycle exercise the NK cell subset (CD16- cells) of mononuclear cells increased significantly. Furthermore an improved interleukin 2 (IL-2) boosting of the NK cell activity was found during work as compared to IFN-alpha and indomethacin-enhanced NK cell activity. These results indicate that NK cells with a high IL-2 response capacity are recruited to the peripheral blood during exercise. The decreased NK cell activity demonstrated 2 h after work was probably not due to fluctuations in size of the NK cell pool, since the proportion of CD16+ cells was normal. The finding that indomethacin fully restored the suppressed NK cell activity in vitro and the demonstration of a twofold increase in monocyte (CD20+ cells) proportions 2 h after work, strongly indicate that prostaglandins released by monocytes during the heavy physical exercise are responsible for the down-regulation of the NK cells.
本研究旨在检测体育锻炼对人类自然杀伤（NK）细胞的影响。六名健康志愿者进行了两项不同的急性体育锻炼测试，间隔至少1周：（1）以最大摄氧量（VO2max）的80%进行60分钟的自行车运动；（2）以VO2max的29%进行60分钟的背部肌肉训练；在运动前、运动最后几分钟期间以及运动后2小时和24小时采集血样。自行车运动期间NK细胞活性（裂解/单核细胞固定数量）增加，2小时后降至最低，并在24小时内恢复到运动前水平。背部肌肉运动对NK细胞活性没有显著影响。骑自行车期间血浆肾上腺素、去甲肾上腺素和皮质醇水平升高，但背部肌肉运动期间未升高，表明运动强度是NK细胞活性的一个决定因素。自行车运动期间单核细胞的NK细胞亚群（CD16-细胞）显著增加。此外，与干扰素-α和吲哚美辛增强的NK细胞活性相比，工作期间发现NK细胞活性的白细胞介素2（IL-2）增强有所改善。这些结果表明，具有高IL-2反应能力的NK细胞在运动期间被募集到外周血中。工作后2小时显示的NK细胞活性降低可能不是由于NK细胞池大小的波动，因为CD16+细胞的比例正常。吲哚美辛在体外完全恢复了受抑制的NK细胞活性这一发现以及工作后2小时单核细胞（CD20+细胞）比例增加两倍的证明，强烈表明剧烈体育锻炼期间单核细胞释放的前列腺素是NK细胞下调的原因。
