Jack C I, Jackson M J, Johnston I D, Hind C R
The University Department of Medicine, University of Liverpool, United Kingdom.
Am J Respir Crit Care Med. 1996 Jun;153(6 Pt 1):1918-23. doi: 10.1164/ajrccm.153.6.8665056.
Serum levels of free radical activity were measured in 37 patients with idiopathic pulmonary fibrosis (IPF) and 16 control subjects. Three assays used were (1) simultaneously measured levels of the 9,11-diene conjugate of linoleic acid and 9,12-linoleic acid expressed as a percent molar ratio (%MR), a measure of free-radical-mediated lipid peroxidation; (2) thiobarbituric acid reactive substances (TBARS), one of which is malondialdehyde; (3) desferrioxamine-chelatable iron assay, a measure of the potential iron available to catalyze free radical generation. Mean %MR, TBARS and desferrioxamine-chelatable iron were all elevated initially in patients with IPF compared with control subjects (%MR, p < 0.0001; TBARS, p = 0.0013; desferrioxamine-chelatable iron, p = 0.0029). Furthermore, the serum %MR was higher in a subset of patients with clinically worsening IPF than in those patients with clinically stable disease (p = 0.002). Treatment did not appear to affect the three different serum indicators of free radical activity. Thus, lipid peroxidation appears to be increased in patients with IPF and is associated with an increase in desferrioxamine-chelatable iron levels. Serum % MR levels appeared to correlate with clinical disease activity, and they may have a role in monitoring disease activity.
对37例特发性肺纤维化（IPF）患者和16名对照者测定了血清自由基活性水平。所采用的三项检测方法为：（1）同时测定亚油酸的9,11 - 二烯共轭物和9,12 - 亚油酸的水平，以摩尔百分比（%MR）表示，这是自由基介导的脂质过氧化的一项指标；（2）硫代巴比妥酸反应性物质（TBARS），其中之一是丙二醛；（3）去铁胺可螯合铁检测，这是对可用于催化自由基生成的潜在铁的一种测定方法。与对照者相比，IPF患者最初的平均%MR、TBARS和去铁胺可螯合铁均升高（%MR，p < 0.0001；TBARS，p = 0.0013；去铁胺可螯合铁，p = 0.0029）。此外，临床病情恶化的IPF患者亚组的血清%MR高于临床病情稳定的患者（p = 0.002）。治疗似乎并未影响自由基活性的这三项不同血清指标。因此，IPF患者的脂质过氧化似乎增加，且与去铁胺可螯合铁水平升高有关。血清%MR水平似乎与临床疾病活动相关，它们可能在监测疾病活动中发挥作用。
