Holmes C, Jones S A, Greenfield S A
University Department of Pharmacology, Oxford, UK.
Brain Res Dev Brain Res. 1995 Sep 29;88(2):212-9. doi: 10.1016/0165-3806(95)00112-q.
The development and regeneration of rat dopaminergic neurons of the ventral mesencephalon was studied in organotypic slice cultures. Single ventral mesencephalon cultures and co-cultures of ventral mesencephalon with striatum (a target region) or cerebellum (a non-target region) were prepared from postnatal day 1 Wistar rats. Cultures were processed for tyrosine hydroxylase and glial fibrillary acidic protein immunoreactivity, at two day intervals, for an overall incubation period of 20 days. Analysis of these cultures revealed that the striatal target tissue, exerted neither a trophic nor a tropic influence on the tyrosine hydroxylase immunoreactive neurons. In both single and co-cultures, tyrosine hydroxylase immunoreactive neurites projected radially from the ventral mesencephalon slice. However, in striatal co-cultures, tyrosine hydroxylase immunoreactive neurites were seen penetrating the striatal slice, whereas in cerebellar co-cultures no tyrosine hydroxylase immunoreactive neurites entered the cerebellar tissue. Glial fibrillary acidic protein positive cells actively migrated from the tissue sections, however tyrosine hydroxylase immunoreactive neurite outgrowth was not guided by these glial cells. Tyrosine hydroxylase immunoreactive neurites terminated once they had penetrated the striatal slice. This retardation of neurite growth by a target region could be important in establishing and reinforcing synaptic connections in the developing nigro-striatal pathway.
在器官型切片培养中研究了大鼠中脑腹侧多巴胺能神经元的发育和再生。从出生后第1天的Wistar大鼠制备中脑腹侧单培养物以及中脑腹侧与纹状体（一个靶区域）或小脑（一个非靶区域）的共培养物。每隔两天对培养物进行酪氨酸羟化酶和胶质纤维酸性蛋白免疫反应性处理，总孵育期为20天。对这些培养物的分析表明，纹状体靶组织对酪氨酸羟化酶免疫反应性神经元既没有营养作用也没有向性影响。在单培养物和共培养物中，酪氨酸羟化酶免疫反应性神经突从中脑腹侧切片呈放射状伸出。然而，在纹状体共培养物中，可见酪氨酸羟化酶免疫反应性神经突穿透纹状体切片，而在小脑共培养物中，没有酪氨酸羟化酶免疫反应性神经突进入小脑组织。胶质纤维酸性蛋白阳性细胞从组织切片中活跃迁移，然而酪氨酸羟化酶免疫反应性神经突的生长不受这些胶质细胞的引导。酪氨酸羟化酶免疫反应性神经突一旦穿透纹状体切片就会终止。靶区域对神经突生长的这种抑制作用在发育中的黑质-纹状体通路中建立和加强突触连接方面可能很重要。
