Alarcón de Noya B, Colmenares C, Losada S, Fermin Z, Masroua G, Ruiz L, Soto L, Noya O
Sección de Biohelmintiasis, Universidad Central de Venezuela, Caracas, Venezuela.
Epidemiol Infect. 1996 Jun;116(3):323-9. doi: 10.1017/s095026880005264x.
In view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15.3% of the total population as determined by SEA-ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.
鉴于肠道寄生虫患者血清与某些曼氏血吸虫抗原有已知的交叉反应性，在委内瑞拉一个非血吸虫病流行区开展了现场工作，使用可溶性虫卵抗原（SEA）的常规免疫酶测定法（ELISA）。通过SEA-ELISA测定，假阳性反应占总人口的15.3%。对假阳性血清进行SEA免疫印迹分析表明，91 kDa和80 kDa的蛋白组分似乎是交叉反应的原因。感染钩虫个体的血清产生交叉反应的频率和强度高于其他血清。105、54、46、42、32、25和15 kDa的SEA组分最具特异性。
