Vargas F S, Wang N S, Teixeira L R, Carmo A O, Silva L M, Light R W
Instituto do Coracao, University of Sao Paulo, Brazil.
Eur Respir J. 1995 Dec;8(12):2174-7. doi: 10.1183/09031936.95.08122174.
Tetracycline has been one of the most commonly used agents for producing a pleurodesis. However, it is no longer available due to more stringent requirements on the manufacturing process. The objective of this project was to determine whether Corynebacterium parvum is an effective sclerosant in an experimental model in rabbits. The following medications were instilled intrapleurally in anaesthetized male rabbits: tetracycline 35 mg.kg-1 or C. parvum 4 or 8 mg, all diluted with bacteriostatic saline solution. Twenty eight days after the instillation, the animals were sacrificed and the pleural spaces assessed macroscopically for evidence of pleurodesis and microscopically for evidence of fibrosis and inflammation. The intrapleural injection of C. parvum was ineffective in creating pleural fibrosis. The mean degree of pleurodesis in the 10 rabbits who received tetracycline was 3.5 +/- 0.7 (scale 0-4) whilst in the 10 rabbits that received 4 mg C. parvum it was 0.0 +/- 0.0, and in the 10 rabbits that received 8 mg C. parvum it was 0.5 +/- 0.8. Based on this study, we recommend that C. parvum should not be used as a pleural sclerosant in patients with normal pleura.
四环素一直是用于产生胸膜固定术的最常用药物之一。然而，由于对生产过程有更严格的要求，它已不再可用。本项目的目的是确定微小棒状杆菌在兔实验模型中是否为一种有效的硬化剂。在麻醉的雄性兔中经胸膜内注入以下药物：35mg·kg-1四环素或4mg或8mg微小棒状杆菌，均用抑菌盐溶液稀释。注入后28天，处死动物，宏观评估胸膜腔有无胸膜固定术的证据，微观评估有无纤维化和炎症的证据。胸膜内注射微小棒状杆菌在造成胸膜纤维化方面无效。接受四环素的10只兔的胸膜固定术平均程度为3.5±0.7（0-4级），而接受4mg微小棒状杆菌的10只兔为0.0±0.0，接受8mg微小棒状杆菌的10只兔为0.5±0.8。基于本研究，我们建议微小棒状杆菌不应作为正常胸膜患者的胸膜硬化剂使用。
