番茄hsc70基因的结构与表达

The structure and expression of an hsc70 gene from Lycopersicon esculentum.

作者信息

Sun S W, Chung M C, Lin T Y

机构信息

Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.

出版信息

Gene. 1996 May 8;170(2):237-41. doi: 10.1016/0378-1119(95)00730-x.

DOI:10.1016/0378-1119(95)00730-x

PMID:8666252

Abstract

A tomato hsc70 genomic clone (Lehsc70-3; Lycopersicon esculentum heat-shock cognate 70-3) was obtained by screening a genomic library with the tomato Lehsc70-2 cDNA. Two restriction fragments of 2.6 and 5 kb, which compose the Lehsc70-3 gene, were subcloned into pBluescriptIIKS+ and analyzed. Transcript mapping reveals that the mature Lehsc70-3 mRNA contains a 122-nt 5' untranslated region (UTR), a coding region of 1956 nt corresponding to a polypeptide of 651 amino acids, an intron of 717 nt and a 3' UTR. Analysis of genomic DNA indicates that Lehsc70-3 is present as a low-copy-number gene. We note that the sequence upstream from the coding region of Lehsc70-3 shares common features with a number of hsc/hsp genes. High-temperature treatment (37 degrees C) caused a twofold increase in the level of the Lehsc70-3 mRNA. However, Lehsc70-3 has also been expressed at substantial levels in tomato vegetative tissues, suggesting a general function of this hsc70 gene in L. esculentum.

摘要

通过用番茄Lehsc70 - 2 cDNA筛选基因组文库，获得了一个番茄hsc70基因组克隆（Lehsc70 - 3；番茄热休克同源蛋白70 - 3）。组成Lehsc70 - 3基因的两个2.6 kb和5 kb的限制性片段被亚克隆到pBluescriptIIKS +中并进行分析。转录图谱显示，成熟的Lehsc70 - 3 mRNA包含一个122 nt的5'非翻译区（UTR）、一个1956 nt的编码区，对应于一个651个氨基酸的多肽、一个717 nt的内含子和一个3' UTR。基因组DNA分析表明，Lehsc70 - 3作为一个低拷贝数基因存在。我们注意到，Lehsc70 - 3编码区上游的序列与许多hsc/hsp基因具有共同特征。高温处理（37℃）导致Lehsc70 - 3 mRNA水平增加两倍。然而，Lehsc70 - 3在番茄营养组织中也有大量表达，表明该hsc70基因在番茄中具有一般功能。