De Giorgi C, De Luca F, Lamberti F
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy.
Gene. 1996 May 8;170(2):261-5. doi: 10.1016/0378-1119(95)00894-2.
A gene (cut-1) coding for a cuticular protein, cuticlin-1, has been isolated and sequenced in the plant parasitic nematode, Meloidogyne artiellia. The nucleotide sequence revealed a typical eukaryotic-like organization, exons and introns bordered by canonical sequences. The 5' flanking region presents nematode-specific sequence motifis, including a trans-splicing signal. Studies on the expression of this gene demonstrated that, while in the adult females cut-1 is not expressed, the removal of the introns occurs in the eggs. These experiments also indicate that cis-splicing precedes the processing of the 5' untranslated region. In no case has a trans-spliced transcript been detected.
在植物寄生线虫南方根结线虫中，已分离并测序了一个编码表皮蛋白角质素-1的基因（cut-1）。核苷酸序列显示出典型的类真核生物结构，外显子和内含子由规范序列界定。5'侧翼区域呈现出线虫特异性的序列基序，包括一个反式剪接信号。对该基因表达的研究表明，虽然在成年雌虫中cut-1不表达，但内含子的去除发生在卵中。这些实验还表明，顺式剪接先于5'非翻译区的加工。在任何情况下都未检测到反式剪接转录本。
