兔源编码钠通道β1亚基的cDNA克隆

Cloning of the cDNA encoding the sodium channel beta 1 subunit from rabbit.

作者信息

Belcher S M, Howe J R

机构信息

Department of Pharmacology Yale University School of Medicine, New Haven CT06520-8066, USA.

出版信息

Gene. 1996 May 8;170(2):285-6. doi: 10.1016/0378-1119(95)00871-3.

DOI:10.1016/0378-1119(95)00871-3

PMID:8666261

Abstract

Here, we report the nucleotide (nt) sequence of the cDNA encoding the sodium channel beta 1 subunit from rabbit (o beta 1). Cloning of the o beta 1 cDNA was accomplished by reverse transcription-polymerase chain reaction using rabbit brain RNA as a template for cDNA synthesis. The nt sequence of the o beta 1 cDNA predicts a 218-amino-acid polypeptide which is 96.3 and 97.3% identical to the beta 1 from human (h beta 1) and rat (r beta 1), respectively.

摘要

在此，我们报道了编码兔钠通道β1亚基（oβ1）的cDNA的核苷酸（nt）序列。通过逆转录-聚合酶链反应，以兔脑RNA作为合成cDNA的模板，完成了oβ1 cDNA的克隆。oβ1 cDNA的nt序列预测其编码一个218个氨基酸的多肽，该多肽与人（hβ1）和大鼠（rβ1）的β1亚基分别有96.3%和97.3%的同源性。