齿瓣兰环斑病毒新加坡分离株的全序列及其与其他烟草花叶病毒属病毒的比较。
The complete sequence of a Singapore isolate of odontoglossum ringspot virus and comparison with other tobamoviruses.
作者信息
Chng C G, Wong S M, Mahtani P H, Loh C S, Goh C J, Kao M C, Chung M C, Watanabe Y
机构信息
Department of Botany, National University of Singapore, Singapore.
出版信息
Gene. 1996 Jun 1;171(2):155-61. doi: 10.1016/0378-1119(96)00046-7.
The complete sequence of a Singapore isolate of odontoglossum ringspot virus (ORSV-S1) comprises 6609 nucleotides (nt) and four open reading frames (ORFs 1 to 4). The 126/183-kDa RNA-dependent RNA polymerase (RdRp), 33-kDa movement protein (MP) and 18-kDa coat protein (CP) cistrons are located at nt 63-3401/4901, 4807-5718, and 5721-6197 on the genome, respectively. The 5' UTR contains three copies of an 8-base direct repeat and (CAA)n motifs. Characteristic tRNA-like structure and three consecutive homologous regions were present in the 3' UTR. The genomic RNA and MP of ORSV-S1 are one of the longest among all members of the TOV group. Phylogenetic analysis of all four genes indicates evolutionary divergence, but within each gene there are some degrees of evolutionary convergence. The conserved amino acid sequences in the MP can be used for the classification of tobamoviruses.
齿舌兰环斑病毒新加坡分离株（ORSV-S1）的完整序列由6609个核苷酸（nt）和四个开放阅读框（ORF 1至4）组成。126/183 kDa的RNA依赖RNA聚合酶（RdRp）、33 kDa的运动蛋白（MP）和18 kDa的外壳蛋白（CP）顺反子分别位于基因组的第63 - 3401/4901 nt、4807 - 5718 nt和5721 - 6197 nt处。5'非翻译区包含三个8碱基直接重复序列和（CAA）n基序的拷贝。3'非翻译区存在特征性的类似tRNA的结构和三个连续的同源区域。ORSV-S1的基因组RNA和MP是烟草花叶病毒组所有成员中最长的之一。对所有四个基因的系统发育分析表明存在进化分歧，但在每个基因内存在一定程度的进化趋同。MP中的保守氨基酸序列可用于烟草花叶病毒的分类。
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