Ying Z, King M L
Department of Cell Biology and Anatomy, School of Medicine, University of Miami, FL 33101, USA.
Gene. 1996 Jun 1;171(2):243-8. doi: 10.1016/0378-1119(95)00891-8.
We have isolated an ortholog (xnov) of the chicken nov gene (for nephroblastoma overexpressed; encoding a putative avian proto-oncogene) from Xenopus laevis (Xl) by screening an Xl ovary cDNA library and genomic library using the entire coding region of human CTGF (encoding connective tissue growth factor) as a probe and by 5'RACE (rapid amplification of cDNA ends). xnov has the same genomic organization as chicken nov, mouse fisp12 and cyr61, but has a unique promoter sequence. The Xl open reading frame (ORF) encodes a 343-amino-acid (aa) polypeptide of 37.9 kDa. Xnov shows 62.9, 60.5, 52.2, 52.1, 47.6 and 45.8% identity with the chicken Nov, human NovH, human CTGF, mouse Fisp12, chicken Cef10 and mouse Cyr61 proteins, respectively. Xnov contains four aa domains which characterize the CTGF family. RT-PCR (reverse transcription-polymerase chain reaction) analysis shows that the xnov mRNA is very low in abundance and appears to be present throughout early Xl development. Our results also indicate that xnov and nov are not orthologs of human CTGF.
我们通过使用人CTGF（编码结缔组织生长因子）的整个编码区作为探针筛选非洲爪蟾（Xl）卵巢cDNA文库和基因组文库，并通过5'RACE（cDNA末端快速扩增），从非洲爪蟾（Xenopus laevis, Xl）中分离出鸡nov基因（肾母细胞瘤过表达；编码一种假定的禽类原癌基因）的直系同源基因（xnov）。xnov与鸡nov、小鼠fisp12和cyr61具有相同的基因组结构，但具有独特的启动子序列。Xl开放阅读框（ORF）编码一个37.9 kDa的343个氨基酸（aa）的多肽。Xnov与鸡Nov、人NovH、人CTGF、小鼠Fisp12、鸡Cef10和小鼠Cyr61蛋白的同一性分别为62.9%、60.5%、52.2%、52.1%、47.6%和45.8%。Xnov包含四个表征CTGF家族的氨基酸结构域。逆转录聚合酶链反应（RT-PCR）分析表明，xnov mRNA丰度非常低，并且似乎在Xl早期发育过程中均有表达。我们的数据还表明，xnov和nov不是人CTGF的直系同源基因。
