Quackenbush J, Davies C, Bailis J M, Khristich J V, Diggle K, Marchuck Y, Tobin J, Clark S P, Rodkins A, Marcano S
Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center at Dallas 75235-8591, USA.
Genomics. 1995 Sep 20;29(2):512-25. doi: 10.1006/geno.1995.9974.
Physical mapping of human chromosomes at a resolution of 100 kb to 1 Mb will provide important reagents for gene identification and framework templates for ultimately determining the complete DNA sequence. Sequence-tagged site (STS) content mapping, coupled with large fragment cloning in yeast artificial chromosomes, provides an efficient mechanism for producing first-generation, low-resolution maps of human chromosomes. Previously, we produced a set of standardized STSs for human chromosome 11 regionally localized by fluorescence in situ hybridization or somatic cell hybrid analysis. In this paper, we used these as well as other STS content, and identify 109 islands spanning an estimated 218 Mb on the 126-Mb chromosome. Since about 62% of the islands contain markers ordered on chromosome 11 by genetic or radiation hybrid analysis, this data set represents a first-order approximation of a physical map of human chromosome 11. This set of clones, contigs, and associated STSs will provide the material for the production of a continuous overlapping set of YACs as well for high-resolution physical mapping based upon sampled and complete DNA sequencing.
以100千碱基对到1兆碱基对的分辨率对人类染色体进行物理图谱绘制，将为基因识别提供重要试剂，并为最终确定完整的DNA序列提供框架模板。序列标签位点（STS）含量图谱绘制，结合酵母人工染色体中的大片段克隆，为生成人类染色体的第一代低分辨率图谱提供了一种有效机制。此前，我们通过荧光原位杂交或体细胞杂交分析，为人类11号染色体制作了一组区域定位的标准化STS。在本文中，我们利用这些以及其他STS含量，在这条126兆碱基对的染色体上识别出109个跨度约218兆碱基对的区域。由于约62%的区域含有通过遗传或辐射杂交分析在11号染色体上排序的标记，该数据集代表了人类11号染色体物理图谱的一阶近似。这组克隆、重叠群和相关的STS将为生成连续重叠的酵母人工染色体组提供材料，也为基于抽样和完整DNA测序的高分辨率物理图谱绘制提供材料。
