Evrard L, Nammour S, Dourov N
Department of Pathology and Electron Microscopy, Medical School, Université Libre de Bruxelles, Belgium.
J Oral Pathol Med. 1996 Feb;25(2):72-7. doi: 10.1111/j.1600-0714.1996.tb00196.x.
An experimental model was established in which the decrease in surface area of CO2 laser wounds on rat tongues was measured during the healing process. Scanning electron microscopy was used to measure and delineate the outlines of the initial wounds and those of the residual wounds. A 50% decrease in the surface area of the wounds was observed 10 days after surgery during the healing process. At day 30, the scar surface area was less than 10% (7.4%) of the wound surface area at day 2. This contrasts with studies reported elsewhere where only minimal contraction was observed during the healing of CO2 laser wounds. Immunoperoxidase techniques revealed that three different phenotypes of myofibroblasts were present inside the healing area, expressing vimentin, actin and desmin. These persisted until day 30 after CO2 irradiation.
建立了一个实验模型，在该模型中测量大鼠舌头二氧化碳激光伤口在愈合过程中表面积的减少情况。使用扫描电子显微镜测量并勾勒初始伤口和残留伤口的轮廓。在愈合过程中，术后10天观察到伤口表面积减少了50%。在第30天，瘢痕表面积小于第2天伤口表面积的10%（7.4%）。这与其他地方报道的研究形成对比，在那些研究中，二氧化碳激光伤口愈合过程中仅观察到最小程度的收缩。免疫过氧化物酶技术显示，愈合区域内存在三种不同表型的肌成纤维细胞，表达波形蛋白、肌动蛋白和结蛋白。这些细胞一直持续到二氧化碳照射后第30天。
