一种来自表皮葡萄球菌的新型质粒，具有对卡那霉素、新霉素和四环素的抗性。

A novel plasmid from Staphylococcus epidermidis specifying resistance to kanamycin, neomycin and tetracycline.

作者信息

Schwarz S, Gregory P D, Werckenthin C, Curnock S, Dyke K G

机构信息

Institut für Kleintierforschung Celle/Merbitz der Bundesforschungsanstalt für Landwirtschaft Braunschweig-Völkenrode (FAL), Germany.

出版信息

J Med Microbiol. 1996 Jul;45(1):57-63. doi: 10.1099/00222615-45-1-57.

DOI:10.1099/00222615-45-1-57

PMID:8667413

Abstract

The naturally occurring plasmid pSTS7 from Staphylococcus epidermidis mediated resistance to tetracycline via a tetL gene and to kanamycin and neomycin via an aadD gene. Plasmid pSTS7 showed partial restriction map and sequence homology to the previously described tetracycline resistance plasmid pNS1981 from Bacillus subtilis and to the kanamycin/neomycin/bleomycin resistance plasmid pUB110 from S. aureus. Sequence analysis of the regions flanking the two resistance genes in pSTS7 led to the identification of a novel site for interplasmid recombination which could explain the derivation of pSTS7 from the incompatible pNS1981- and pUB110-like parental plasmids under tetracycline-selective pressure.

摘要

来自表皮葡萄球菌的天然质粒pSTS7通过tetL基因介导对四环素的抗性，并通过aadD基因介导对卡那霉素和新霉素的抗性。质粒pSTS7与先前描述的来自枯草芽孢杆菌的四环素抗性质粒pNS1981以及来自金黄色葡萄球菌的卡那霉素/新霉素/博来霉素抗性质粒pUB110显示出部分限制性图谱和序列同源性。对pSTS7中两个抗性基因侧翼区域的序列分析导致鉴定出一个新的质粒间重组位点，这可以解释在四环素选择压力下pSTS7从不相容的pNS1981样和pUB110样亲本质粒衍生而来的原因。