多重耐药金黄色葡萄球菌分离株中耐药决定因子的转移。

Transfer of resistance determinants from a multi-resistant Staphylococcus aureus isolate.

作者信息

Udo E E, Grubb W B

机构信息

School of Medical Technology, Curtin University of Technology, Perth, Western Australia.

出版信息

J Med Microbiol. 1991 Aug;35(2):72-9. doi: 10.1099/00222615-35-2-72.

DOI:10.1099/00222615-35-2-72

PMID:1875394

Abstract

The clinical isolate Staphylococcus aureus WBG1024 was resistant to cadmium, benzyl penicillin, kanamycin, neomycin, streptomycin, tetracycline and trimethoprim and harboured a conjugative plasmid pWBG637 (34.5 kb) and non-conjugative plasmids of 23.8, 4.4, 2.8 and 1.9 kb. Transduction and mixed-culture transfer experiments demonstrated that the 4.4-kb plasmid (pWBG632) encoded resistance to tetracycline and the 23.8-kb plasmid (pWBG628) encoded resistance to cadmium, benzyl penicillin, kanamycin, neomycin and streptomycin. The conjugative plasmid pWBG637 was able to mobilise a further 4.4-kb plasmid (pWBG633) encoding streptomycin resistance and recombined with the multiresistance plasmid pWBG628 to produce transconjugantes of various resistance phenotypes.

摘要

临床分离株金黄色葡萄球菌WBG1024对镉、苄青霉素、卡那霉素、新霉素、链霉素、四环素和甲氧苄啶耐药，携带一个接合质粒pWBG637（34.5 kb）以及大小分别为23.8、4.4、2.8和1.9 kb的非接合质粒。转导和混合培养转移实验表明，4.4 kb质粒（pWBG632）编码对四环素的耐药性，23.8 kb质粒（pWBG628）编码对镉、苄青霉素、卡那霉素、新霉素和链霉素的耐药性。接合质粒pWBG637能够转移另一个编码链霉素耐药性的4.4 kb质粒（pWBG633），并与多耐药性质粒pWBG628重组，产生具有各种耐药表型的转接合子。