HeLa细胞中血质体形成和血红蛋白生物合成的诱导。

Induction of hemosome formation and hemoglobin biosynthesis in HeLa cells.

作者信息

Brunner A, de Rizzo E, Mitsutani C Y, Ribeiro de Barros M A

出版信息

Res Exp Med (Berl). 1977 Apr 29;169(1):17-22. doi: 10.1007/BF01852115.

Abstract

Tissue cultures of HeLa cells in Hank's medium suspension, previously mixed with a solution containing reticulocyte polysomes and/or free globin, hemoglobin and blood plasma from bled rabbits, were homogenized, and an electrophoretic pattern of hemoglobin band was obtained from the supernatant; hemoglobin denatures under the tissue culture conditions. HeLa cells show typical hemosomes, identidical to those found in immature erythrocytes of embryos, and adult mammals with hemolytic anemias. When HeLa cell suspensions are mixed with solutions void of anemic blood plasma, or containing normal rabbit blood plasma, anomalous organelles are formed in the cells, and no hemoglobin biosynthesis occurs.

摘要

将先前与含有网织红细胞多核糖体和/或游离珠蛋白、血红蛋白以及放血兔血浆的溶液混合的汉克氏培养基悬浮液中的海拉细胞组织培养物进行匀浆处理，然后从上清液中获得血红蛋白带的电泳图谱；血红蛋白在组织培养条件下会变性。海拉细胞显示出典型的血球体，与在胚胎未成熟红细胞以及患有溶血性贫血的成年哺乳动物中发现的血球体相同。当海拉细胞悬浮液与不含贫血血浆的溶液或含有正常兔血浆的溶液混合时，细胞中会形成异常细胞器，且不会发生血红蛋白生物合成。