Del Gatto F, Gesnel M C, Breathnach R
INSERM U211, Institut de Biologie, Nantes, France.
Nucleic Acids Res. 1996 Jun 1;24(11):2017-21. doi: 10.1093/nar/24.11.2017.
The fibroblast growth factor receptor-2 gene contains a pair of alternative exons, K-SAM and BEK, which are spliced in a cell type specific manner. We have shown previously that a 10 nucleotide sequence within the K-SAM exon exerts a negative effect on K-SAM exon splicing independent of cell type. We demonstrate here that this sequence works autonomously, as it can repress splicing of a heterologous exon, the EIIIb alternative exon of the rat fibronectin gene. By introducing point mutations into the 10 nucleotide sequence, we have shown that the functional portion is limited to 4 nucleotides, TAGG, the dinucleotide AG of which is particularly important. This short sequence may participate in the control of splicing of exons carrying it, provided that they carry weak splice sites.
成纤维细胞生长因子受体-2基因包含一对可变外显子，即K-SAM和BEK，它们以细胞类型特异性方式进行剪接。我们之前已经表明，K-SAM外显子内的一个10个核苷酸的序列对K-SAM外显子的剪接产生负面影响，且与细胞类型无关。我们在此证明该序列具有自主作用，因为它可以抑制异源外显子（大鼠纤连蛋白基因的EIIIb可变外显子）的剪接。通过将点突变引入该10个核苷酸的序列，我们发现功能部分仅限于4个核苷酸，即TAGG，其中的二核苷酸AG尤为重要。这个短序列可能参与携带它的外显子的剪接控制，前提是这些外显子携带弱剪接位点。
