外显子序列TAGG可抑制剪接。
The exon sequence TAGG can inhibit splicing.
作者信息
Del Gatto F, Gesnel M C, Breathnach R
机构信息
INSERM U211, Institut de Biologie, Nantes, France.
出版信息
Nucleic Acids Res. 1996 Jun 1;24(11):2017-21. doi: 10.1093/nar/24.11.2017.
Abstract
The fibroblast growth factor receptor-2 gene contains a pair of alternative exons, K-SAM and BEK, which are spliced in a cell type specific manner. We have shown previously that a 10 nucleotide sequence within the K-SAM exon exerts a negative effect on K-SAM exon splicing independent of cell type. We demonstrate here that this sequence works autonomously, as it can repress splicing of a heterologous exon, the EIIIb alternative exon of the rat fibronectin gene. By introducing point mutations into the 10 nucleotide sequence, we have shown that the functional portion is limited to 4 nucleotides, TAGG, the dinucleotide AG of which is particularly important. This short sequence may participate in the control of splicing of exons carrying it, provided that they carry weak splice sites.
摘要
成纤维细胞生长因子受体-2基因包含一对可变外显子，即K-SAM和BEK，它们以细胞类型特异性方式进行剪接。我们之前已经表明，K-SAM外显子内的一个10个核苷酸的序列对K-SAM外显子的剪接产生负面影响，且与细胞类型无关。我们在此证明该序列具有自主作用，因为它可以抑制异源外显子（大鼠纤连蛋白基因的EIIIb可变外显子）的剪接。通过将点突变引入该10个核苷酸的序列，我们发现功能部分仅限于4个核苷酸，即TAGG，其中的二核苷酸AG尤为重要。这个短序列可能参与携带它的外显子的剪接控制，前提是这些外显子携带弱剪接位点。
相似文献
1
The exon sequence TAGG can inhibit splicing.外显子序列TAGG可抑制剪接。
Nucleic Acids Res. 1996 Jun 1;24(11):2017-21. doi: 10.1093/nar/24.11.2017.
2
Polypyrimidine tract-binding protein represses splicing of a fibroblast growth factor receptor-2 gene alternative exon through exon sequences.聚嘧啶序列结合蛋白通过外显子序列抑制成纤维细胞生长因子受体2基因可变外显子的剪接。
J Biol Chem. 2001 Nov 23;276(47):43677-87. doi: 10.1074/jbc.M107381200. Epub 2001 Sep 13.
3
Control of BEK and K-SAM splice sites in alternative splicing of the fibroblast growth factor receptor 2 pre-mRNA.成纤维细胞生长因子受体2前体信使核糖核酸可变剪接中BEK和K-SAM剪接位点的调控
Mol Cell Biol. 1993 Sep;13(9):5461-8. doi: 10.1128/mcb.13.9.5461-5468.1993.
4
Exon and intron sequences, respectively, repress and activate splicing of a fibroblast growth factor receptor 2 alternative exon.外显子和内含子序列分别抑制和激活成纤维细胞生长因子受体2可变外显子的剪接。
Mol Cell Biol. 1995 Sep;15(9):4825-34. doi: 10.1128/MCB.15.9.4825.
5
Multiple interdependent sequence elements control splicing of a fibroblast growth factor receptor 2 alternative exon.多个相互依赖的序列元件控制成纤维细胞生长因子受体2可变外显子的剪接。
Mol Cell Biol. 1997 Sep;17(9):5106-16. doi: 10.1128/MCB.17.9.5106.
6
Elements regulating an alternatively spliced exon of the rat fibronectin gene.调控大鼠纤连蛋白基因可变剪接外显子的元件。
Mol Cell Biol. 1993 Sep;13(9):5301-14. doi: 10.1128/mcb.13.9.5301-5314.1993.
7
A novel intronic cis element, ISE/ISS-3, regulates rat fibroblast growth factor receptor 2 splicing through activation of an upstream exon and repression of a downstream exon containing a noncanonical branch point sequence.一种新型内含子顺式元件ISE/ISS-3，通过激活上游外显子和抑制包含非经典分支点序列的下游外显子来调节大鼠成纤维细胞生长因子受体2的剪接。
Mol Cell Biol. 2005 Jan;25(1):250-63. doi: 10.1128/MCB.25.1.250-263.2005.
8
In vitro splicing of fibronectin pre-mRNAs.纤连蛋白前体信使核糖核酸的体外剪接
Nucleic Acids Res. 1990 Jul 25;18(14):4089-97. doi: 10.1093/nar/18.14.4089.
9
Regulation of alternative pre-mRNA splicing by a novel repeated hexanucleotide element.一种新型重复六核苷酸元件对可变前体mRNA剪接的调控
Genes Dev. 1994 Jul 1;8(13):1561-74. doi: 10.1101/gad.8.13.1561.
10
A Non-sequence-specific double-stranded RNA structural element regulates splicing of two mutually exclusive exons of fibroblast growth factor receptor 2 (FGFR2).一种非序列特异性双链RNA结构元件调节成纤维细胞生长因子受体2（FGFR2）两个相互排斥外显子的剪接。
J Biol Chem. 2002 Dec 20;277(51):50143-54. doi: 10.1074/jbc.M207409200. Epub 2002 Oct 21.
引用本文的文献
1
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.相互排斥的剪接通过涉及三个不同剪接调控元件及其配体的组合机制来调节 Nav1.6 钠离子通道的功能。
Nucleic Acids Res. 2012 Jul;40(13):6255-69. doi: 10.1093/nar/gks249. Epub 2012 Mar 19.
2
Combined use of MS2 and PP7 coat fusions shows that TIA-1 dominates hnRNP A1 for K-SAM exon splicing control.MS2和PP7外壳融合蛋白的联合使用表明，在K-SAM外显子剪接控制中，TIA-1比hnRNP A1起主导作用。
J Biomed Biotechnol. 2009;2009:104853. doi: 10.1155/2009/104853. Epub 2010 Jan 14.
3
Regions of the amino terminus of the P2X receptor required for modification by phorbol ester and mGluR1alpha receptors.佛波酯和代谢型谷氨酸受体1α受体修饰所需的P2X受体氨基末端区域。
J Neurochem. 2009 Jan;108(2):331-40. doi: 10.1111/j.1471-4159.2008.05761.x. Epub 2008 Nov 22.
4
Multiple and specific mRNA processing targets for the major human hnRNP proteins.主要人类核不均一核糖核蛋白（hnRNP）的多种特异性mRNA加工靶点。
Mol Cell Biol. 2008 Oct;28(19):6033-43. doi: 10.1128/MCB.00726-08. Epub 2008 Jul 21.
5
Identification of an intronic splicing enhancer essential for the inclusion of FGFR2 exon IIIc.鉴定对于成纤维细胞生长因子受体2（FGFR2）外显子IIIc包含至关重要的内含子剪接增强子。
J Biol Chem. 2008 Apr 11;283(15):10058-67. doi: 10.1074/jbc.M800087200. Epub 2008 Feb 6.
6
Modulating role of RNA structure in alternative splicing of a critical exon in the spinal muscular atrophy genes.RNA结构在脊髓性肌萎缩症基因关键外显子可变剪接中的调控作用
Nucleic Acids Res. 2007;35(2):371-89. doi: 10.1093/nar/gkl1050. Epub 2006 Dec 14.
7
Imaging the alternative silencing of FGFR2 exon IIIb in vivo.体内成像FGFR2外显子IIIb的选择性沉默。
RNA. 2006 Dec;12(12):2073-9. doi: 10.1261/rna.248506. Epub 2006 Oct 26.
8
Alternative inclusion of fibroblast growth factor receptor 2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity.在邓宁前列腺肿瘤中，成纤维细胞生长因子受体2外显子IIIc的选择性包含揭示了意想不到的上皮-间质可塑性。
Proc Natl Acad Sci U S A. 2006 Sep 19;103(38):14116-21. doi: 10.1073/pnas.0603090103. Epub 2006 Sep 8.
9
Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice.Fox-2介导上皮细胞特异性成纤维细胞生长因子受体2外显子的选择。
Mol Cell Biol. 2006 Feb;26(4):1209-22. doi: 10.1128/MCB.26.4.1209-1222.2006.
10
A combinatorial code for splicing silencing: UAGG and GGGG motifs.一种用于剪接沉默的组合密码：UAGG和GGGG基序。
PLoS Biol. 2005 May;3(5):e158. doi: 10.1371/journal.pbio.0030158. Epub 2005 Apr 19.
本文引用的文献
1
Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein A1 and pre-mRNA splicing factor SF2/ASF.异质性核糖核蛋白A1和前体mRNA剪接因子SF2/ASF对外显子跳跃和包含的调控
Mol Cell Biol. 1993 May;13(5):2993-3001. doi: 10.1128/mcb.13.5.2993-3001.1993.
2
The skipping of constitutive exons in vivo induced by nonsense mutations.无义突变在体内诱导组成型外显子跳跃。
Science. 1993 Jan 29;259(5095):680-3. doi: 10.1126/science.8430317.
3
Structural and functional diversity in the FGF receptor multigene family.成纤维细胞生长因子受体多基因家族中的结构与功能多样性。
Adv Cancer Res. 1993;60:1-41. doi: 10.1016/s0065-230x(08)60821-0.
4
Mutation of an RSV intronic element abolishes both U11/U12 snRNP binding and negative regulation of splicing.呼吸道合胞病毒内含子元件的突变会消除U11/U12核小核糖核蛋白的结合以及剪接的负调控。
Genes Dev. 1993 Oct;7(10):1926-36. doi: 10.1101/gad.7.10.1926.
5
Developmental localization of the splicing alternatives of fibroblast growth factor receptor-2 (FGFR2).成纤维细胞生长因子受体2（FGFR2）剪接异构体的发育定位
Dev Biol. 1993 Aug;158(2):475-86. doi: 10.1006/dbio.1993.1205.
6
The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element.心肌肌钙蛋白T可变外显子包含一个新的富含嘌呤的正性剪接元件。
Mol Cell Biol. 1993 Jun;13(6):3660-74. doi: 10.1128/mcb.13.6.3660-3674.1993.
7
Control of BEK and K-SAM splice sites in alternative splicing of the fibroblast growth factor receptor 2 pre-mRNA.成纤维细胞生长因子受体2前体信使核糖核酸可变剪接中BEK和K-SAM剪接位点的调控
Mol Cell Biol. 1993 Sep;13(9):5461-8. doi: 10.1128/mcb.13.9.5461-5468.1993.
8
Polypurine sequences within a downstream exon function as a splicing enhancer.下游外显子内的聚嘌呤序列作为剪接增强子发挥作用。
Mol Cell Biol. 1994 Feb;14(2):1347-54. doi: 10.1128/mcb.14.2.1347-1354.1994.
9
General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer.通用剪接因子SF2/ASF通过与外显子剪接增强子结合来促进可变剪接。
Genes Dev. 1993 Dec;7(12B):2598-608. doi: 10.1101/gad.7.12b.2598.
10
A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding.人类纤连蛋白可变ED1外显子中的一个剪接增强子与SR蛋白相互作用，并刺激U2 snRNP结合。
Genes Dev. 1993 Dec;7(12A):2405-17. doi: 10.1101/gad.7.12a.2405.
Zotero 插件