Eloranta M L, Sandberg K, Alm G V
Division of Immunology, Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.
Scand J Immunol. 1996 Apr;43(4):356-60. doi: 10.1046/j.1365-3083.1996.d01-62.x.
Murine mononuclear leucocytes from bone marrow, spleen, lymph node and blood stimulated in vitro by UV-irradiated herpes simplex type I virus (HSV) produced about equal proportions of IFN-alpha and -beta determined by immunoassay. Thymocytes produced only IFN-alpha. The frequency of IFN-alpha/beta mRNA containing cells detected by in situ hybridization was highest with bone marrow (15 per 10(4) cells), followed by spleen (4/10(4)), lymph node (2/10(4)), blood (1/10(4)) and thymus (0.2/10(4)). Such IFN-alpha/beta producing cells (IPCs) were heavily labelled in autoradiographs, each producing about 0.4 U of IFN. After one intravenous injection of UV-irradiated HSV in mice, high levels of IFN-alpha and -beta were present in blood at 3-9h and little or none at 24h or later. Frequent cells strongly positive for IFN-alpha mRNA at in situ hybridization and for IFN-alpha/beta at immunohistochemical staining were found almost exclusively in the marginal zones of spleens. Occasional IPCs were detected in lymph nodes but not in bone marrow, liver and kidneys. The marginal zone IPCs may be the major source of IFN in blood, and high splenic levels of IFN-alpha/beta should have efficient antiviral and immunoregulatory functions.
来自骨髓、脾脏、淋巴结和血液的小鼠单核白细胞，在体外经紫外线照射的I型单纯疱疹病毒（HSV）刺激后，通过免疫测定法测定，产生的α干扰素和β干扰素比例大致相等。胸腺细胞仅产生α干扰素。通过原位杂交检测到的含α/β干扰素mRNA的细胞频率，以骨髓最高（每10⁴个细胞中有15个），其次是脾脏（4/10⁴）、淋巴结（2/10⁴）、血液（1/10⁴）和胸腺（0.2/10⁴）。这些产生α/β干扰素的细胞（IPC）在放射自显影片中被大量标记，每个细胞产生约0.4单位的干扰素。给小鼠静脉注射一次紫外线照射的HSV后，血液中在3 - 9小时出现高水平的α干扰素和β干扰素，在24小时及以后则很少或没有。原位杂交时α干扰素mRNA呈强阳性且免疫组织化学染色时α/β干扰素呈强阳性的细胞，几乎只在脾脏的边缘区发现。在淋巴结中偶尔检测到IPC，但在骨髓、肝脏和肾脏中未检测到。边缘区IPC可能是血液中干扰素的主要来源，脾脏中高水平的α/β干扰素应具有有效的抗病毒和免疫调节功能。
