Riffault S, Eloranta M L, Carrat C, Sandberg K, Charley B, Alm G
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
J Interferon Cytokine Res. 1996 Dec;16(12):1007-14. doi: 10.1089/jir.1996.16.1007.
As in vivo experimental system involving the local induction of interferon-alpha/beta (IFN-alpha/beta) responses was established in mice by injecting s.c. ultraviolet (UV)-inactivated herpes simplex virus (HSV) in the right ears, the left ears receiving phosphate-buffered saline (PBS) as a control. Circulating IFN-alpha/beta was present in blood as early as 6 h postinjection, and little or none was found 24 h postinjection. Identification of IFN-alpha/beta-producing cells, carried out by immunohistochemistry and in situ hybridization, demonstrated that the IFN response occurred mainly in the lymph node draining the HSV-injected ear and not in the contralateral lymph node. Occasionally, IFN-alpha/beta-producing cells were found in the spleen and in the skin. The injected HSV caused an inflammatory reaction in the skin and an almost threefold enlargement of the draining lymph node within 6 h. The latter was characterized by a general accumulation of all major lymphocyte subsets and a striking infiltration of neutrophils. Injection s.c. of neutralizing anti-IFN-alpha/beta antibodies before HSV injection reduced the increase in size of the draining lymph node by approximately 50% at 6 h, and no significant effects were seen at 24 h. The localization of cells producing IFN-alpha/beta in the lymph node and the capacity of such IFN-alpha/beta to at least partially mediate an early accumulation of cells suggest that the local IFN-alpha/beta response may have an important role in the initiation of early antiviral immune responses.
通过在小鼠右耳皮下注射紫外线（UV）灭活的单纯疱疹病毒（HSV），建立了一种涉及局部诱导α/β干扰素（IFN-α/β）反应的体内实验系统，左耳注射磷酸盐缓冲盐水（PBS）作为对照。注射后6小时血液中就出现了循环的IFN-α/β，而注射后24小时则很少或没有发现。通过免疫组织化学和原位杂交对产生IFN-α/β的细胞进行鉴定，结果表明IFN反应主要发生在引流注射HSV耳朵的淋巴结中，而对侧淋巴结中则未发生。偶尔，在脾脏和皮肤中也发现了产生IFN-α/β的细胞。注射的HSV在6小时内引起皮肤炎症反应，并使引流淋巴结肿大近三倍。后者的特征是所有主要淋巴细胞亚群普遍聚集，中性粒细胞显著浸润。在注射HSV之前皮下注射中和性抗IFN-α/β抗体，在6小时时可使引流淋巴结的增大减少约50%，而在24小时时未观察到显著影响。产生IFN-α/β的细胞在淋巴结中的定位以及这种IFN-α/β至少部分介导细胞早期聚集的能力表明，局部IFN-α/β反应可能在早期抗病毒免疫反应的启动中起重要作用。
