Gu K, Kin S, Saitoh Y, Nosaka S, Sasaki T, Yamauchi M, Nakayama K
First Department of Surgery, Shimane Medical University, Izumo, Japan.
Transplantation. 1996 Jun 15;61(11):1572-5. doi: 10.1097/00007890-199606150-00004.
We compared the efficacy of using histidine-tryptophan-ketoglurate (HTK) solution with that of University of Wisconsin (UW) solution for heart preservation in an isolated rat heart preparation. Nicorandil (NCR) exerts its action as an ATP-sensitive potassium channel opener at low extracellular potassium concentrations, and HTK solution has a low potassium concentration. Therefore, we also investigated the efficacy of using HTK solution with NCR following 12-hr preservation. Hearts isolated from male Wistar rats were mounted on a Langendorff apparatus to estimate baseline aortic flow (AF), coronary flow (CF), cardiac out-put (CO), heart rate (HR), systolic pressure (SP), aortic mean pressure, and the rate-pressure product (RPP). The hearts were divided into four groups: group 1, 8-hr storage in UW solution; groups 2 and 3, 8- or 12-hr storage in HTK solution, respectively; and group 4, 12-hr storage in HTK solution with NCR. They were arrested and stored at 4 degrees C in each preservation solution. Following storage, they were reperfused and postpreservative function was measured to assess cardiac functional recovery. Concentrations of creatine phosphokinase, troponin-T, and lactate in the coronary perfusate were measured. Frozen tissue samples from groups 3 and 4 were analyzed for adenylate content and cGMP. The myocardial water content was also measured. The recovery of AF, CF, CO, SP, and RPP in group 2 was significantly improved compared with that in group 1 (P<0.05). The recovery of AF, CF, CO and HR in group 4 was significantly better than that in group 3 (P<0.05). Creatine phosphokinase leakage in group 2 and troponin-T leakage in group 4 were significantly reduced (P<0.05 vs. groups 1 and 3, respectively). Total adenine nucleotides and the adenylate energy charge in group 4 were well sustained (P<0.05 vs. group 3). These results suggest that HTK solution is more effective than UW solution for cardiac preservation, and that NCR provides still better protection.
我们在离体大鼠心脏制备中比较了组氨酸 - 色氨酸 - 酮戊二酸（HTK）溶液与威斯康星大学（UW）溶液用于心脏保存的效果。尼可地尔（NCR）在低细胞外钾浓度下作为ATP敏感性钾通道开放剂发挥作用，而HTK溶液钾浓度较低。因此，我们还研究了保存12小时后使用HTK溶液加NCR的效果。从雄性Wistar大鼠分离的心脏安装在Langendorff装置上，以评估基线主动脉流量（AF）、冠状动脉流量（CF）、心输出量（CO）、心率（HR）、收缩压（SP）、主动脉平均压和速率 - 压力乘积（RPP）。心脏分为四组：第1组，在UW溶液中保存8小时；第2组和第3组，分别在HTK溶液中保存8或12小时；第4组，在含NCR的HTK溶液中保存12小时。它们在每种保存溶液中于4℃停搏并保存。保存后，进行再灌注并测量保存后功能以评估心脏功能恢复。测量冠状动脉灌注液中肌酸磷酸激酶、肌钙蛋白 - T和乳酸的浓度。对第3组和第4组的冷冻组织样本进行腺苷酸含量和环鸟苷酸分析。还测量了心肌含水量。与第1组相比，第2组中AF、CF、CO、SP和RPP的恢复显著改善（P<0.05）。第4组中AF、CF、CO和HR的恢复明显优于第3组（P<0.05）。第2组中肌酸磷酸激酶泄漏和第4组中肌钙蛋白 - T泄漏显著减少（分别与第1组和第3组相比，P<0.05）。第4组中总腺嘌呤核苷酸和腺苷酸能量电荷维持良好（与第3组相比，P<0.05）。这些结果表明，HTK溶液在心脏保存方面比UW溶液更有效，并且NCR提供了更好的保护。
