Svetlova M P, Kazakov V I, Timchenko D I, Korshunova Iu O, Bozhkov V M, Tomilin N V
Tsitologiia. 1995;37(8):813-9.
16 pairs of oligonucleotide primers, complementary to unique DNA sequences of human chromosome 3, were synthesized. For 10 of these, fragments of expected length were generated in polymerase chain reaction (PCR). These fragments may be used as markers for detailed physical mapping of this chromosome. The above primers were used in PCR in order to analyse a hybrid mice-human cell line which contained presumably a fragment of human chromosome 3. The presence of human DNA in the hybrid line has been shown, but no ultimate evidence was received to confirm its location in human chromosome 3. By means of primers, complementary to the butyrylcholinesterase gene (BCHE), pools of clones from the yeast total library of human DNA were analysed, and then the pool and later the individual [correction of undividual] clone, containing a fragment of BCHE gene, were identified.
合成了16对与人类3号染色体独特DNA序列互补的寡核苷酸引物。其中10对引物在聚合酶链反应（PCR）中产生了预期长度的片段。这些片段可用作该染色体详细物理图谱的标记。上述引物用于PCR，以分析一个可能含有人类3号染色体片段的杂种小鼠-人类细胞系。已证明杂种细胞系中存在人类DNA，但未获得确凿证据来证实其在人类3号染色体上的位置。通过与丁酰胆碱酯酶基因（BCHE）互补的引物，分析了来自人类DNA酵母总文库的克隆池，然后鉴定出含有BCHE基因片段的克隆池以及随后的单个克隆。
