Arnold J P, Haeger M, Bengtson J P, Bengtsson A, Lisander B
Department of Anaesthesiology & Intensive Care, Sahlgrenska University Hospital, Goteborg, Sweden.
Anaesth Intensive Care. 1995 Dec;23(6):683-6. doi: 10.1177/0310057X9502300604.
Ten patients undergoing hip replacement surgery were studied regarding activation of complement and leukocytes in association with collection of wound drainage blood. The blood was collected postoperatively but not reinfused due to the possible risks with reinfusion of blood containing inflammatory mediators. Blood samples for analysis of complement activation (TCC), leukocyte activation (PMN elastase) and cytokines (Interleukin-6) were drawn preoperatively from the patients. Blood samples were also drawn intraoperatively from the wound. Samples were also drawn from the collected wound drainage blood, before and after blood was passed through a microporous filter. There were elevated concentrations of TCC, PMN elastase and IL-6 in the collected wound drainage blood before and after the filter. The filtration did not significantly reduce the concentrations of these factors. In the wound blood the concentrations were higher compared to those found in the systemic blood preoperatively, but lower compared to concentrations found in the collected drainage blood. The study demonstrates that the collection of wound drainage whole blood is associated with activation of complement, release of PMN elastase and cytokines.
对10名接受髋关节置换手术的患者进行了研究，以探讨补体和白细胞的激活与伤口引流血液采集之间的关系。血液在术后采集，但由于回输含有炎症介质的血液可能存在风险，故未进行回输。术前从患者身上采集用于分析补体激活（总补体）、白细胞激活（中性粒细胞弹性蛋白酶）和细胞因子（白细胞介素-6）的血样。术中也从伤口采集血样。在血液通过微孔过滤器前后，还从收集的伤口引流血液中取样。过滤器前后收集的伤口引流血液中总补体、中性粒细胞弹性蛋白酶和白细胞介素-6的浓度均升高。过滤并未显著降低这些因子的浓度。伤口血液中的浓度与术前全身血液中的浓度相比更高，但与收集的引流血液中的浓度相比更低。该研究表明，伤口引流全血的采集与补体激活、中性粒细胞弹性蛋白酶释放和细胞因子有关。
