Goto Y, Nishino I, Horai S, Nonaka I
Department of Ultrastructural Research, National Institute of Neuroscience, Tokyo, Japan.
Biochem Biophys Res Commun. 1996 May 15;222(2):215-9. doi: 10.1006/bbrc.1996.0724.
Deletions and occasional duplications in mitochondrial DNA have been known to be present in mitochondrial diseases and in aged tissues. The junctional sequences of the rearrangements must be determined for detecting duplication, but its procedures seem laborious for routine examination. The joint method of long polymerase chain reaction plus digestion by three restriction enzymes provides a simple method to detect and map the deletion sites of mitochondrial DNA.
线粒体疾病和衰老组织中存在线粒体DNA的缺失以及偶尔的重复现象。为了检测重复，必须确定重排的连接序列，但其操作程序对于常规检查而言似乎很繁琐。长聚合酶链反应结合三种限制性酶消化的联合方法提供了一种检测和定位线粒体DNA缺失位点的简单方法。
