大鼠支持细胞中外切ATP酶活性的鉴定与特性分析。
Identification and characterization of an ecto-ATPase activity in rat Sertoli cells.
作者信息
Barbacci E, Filippini A, De Cesaris P, Ziparo E
机构信息
Institute of Histology and General Embryology, School of Medicine, La Sapienza University of Rome, Italy.
出版信息
Biochem Biophys Res Commun. 1996 May 15;222(2):273-9. doi: 10.1006/bbrc.1996.0734.
A membrane associated extracellular ATPase (ecto) has been identified on rat Sertoli cells. Sertoli cell ecto-ATPase demonstrated a Km for ATP of 114 muM and a V(max) of 1.79 mumol/min/2 x 10(5) cells and was activated by either Mg2+ or Ca2+. This ecto-ATPase hydrolyzes other nucleoside triphosphates, but is inactive with ADP. The effects of some possible inhibitors of ectonucleotidases on the breakdown of extracellular ATP by Sertoli cells were also investigated.
在大鼠支持细胞上已鉴定出一种与膜相关的细胞外ATP酶（ecto）。支持细胞ecto-ATP酶对ATP的Km值为114μM，V(max)为1.79μmol/分钟/2×10(5)个细胞，并被Mg2+或Ca2+激活。这种ecto-ATP酶能水解其他核苷三磷酸，但对ADP无活性。还研究了一些可能的外核苷酸酶抑制剂对支持细胞分解细胞外ATP的影响。
Zotero 插件