Doevendans P A, Becker K D, An R H, Kass R S
Department of Cardiology, Cardiovascular Research Institute Maastricht, The Netherlands.
Biochem Biophys Res Commun. 1996 May 15;222(2):352-8. doi: 10.1006/bbrc.1996.0747.
In vivo reporter genes can be used in different ways in molecular cardiology. In this paper studies are presented using the green fluorescent protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identification prior to patch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells.
体内报告基因可在分子心脏病学中以不同方式使用。本文介绍了使用绿色荧光蛋白及其一种突变体S65T-GFP作为体内报告基因的研究。借助这种新的分子工具，我们研究了小鼠心室肌球蛋白轻链2启动子的细胞类型特异性、膜片钳操作前的阳性细胞鉴定以及对瞬时转染的哺乳动物细胞进行荧光激活细胞分选的应用。
