Ray-Gallet D, Tavitian A, Moreau-Gachelin F
INSERM U.248, Institut Curie, Paris, France.
Biochem Biophys Res Commun. 1996 Jun 14;223(2):257-63. doi: 10.1006/bbrc.1996.0881.
Spi-B is an Ets transcription factor related to the oncoprotein Spi-1/PU.1 and highly expressed in B lymphoid cells. The Ets proteins share a conserved Ets domain that mediates specific DNA binding. Spi-B binds DNA sequences containing a core 5'-GGAA-3' and activates transcription through this motif. Up to date, the biological function of Spi-B remains unknown. Here, we describe the characterization of an alternatively spliced variant of Spi-B, named deltaSpi-B, which has lost the Ets domain. In B lymphoid cells, deltaspi-B and spi-B mRNAs were present simultaneously in a ratio of around 10%. DeltaSpi-B product was not able to bind DNA and was recovered in cytoplasmic cellular extracts. We raise the hypothesis that delta Spi-B might affect Spi-B function by recruiting factors involved in Spi-B activity.
Spi-B是一种与癌蛋白Spi-1/PU.1相关的Ets转录因子，在B淋巴细胞中高度表达。Ets蛋白共享一个保守的Ets结构域，该结构域介导特异性DNA结合。Spi-B结合含有核心序列5'-GGAA-3'的DNA序列，并通过该基序激活转录。迄今为止，Spi-B的生物学功能仍不清楚。在这里，我们描述了一种名为deltaSpi-B的Spi-B可变剪接变体的特征，它已经失去了Ets结构域。在B淋巴细胞中，deltaspi-B和spi-B mRNA同时存在，比例约为10%。DeltaSpi-B产物不能结合DNA，并且在细胞质细胞提取物中被检测到。我们提出一个假设，即delta Spi-B可能通过招募参与Spi-B活性的因子来影响Spi-B的功能。
