Jadot M, Wattiaux R, Mainferme F, Dubois F, Claessens A, Wattiaux-De Coninck S
Laboratoire de Chimie Physiologique, Facultes Universitairés Notre Dame de la Paix, Namur, Belgium.
Biochem Biophys Res Commun. 1996 Jun 14;223(2):353-9. doi: 10.1006/bbrc.1996.0898.
Lamp II (for lysosomal associated membrane protein II) is an integral type I glycoprotein. It consists of a very large and heavily glycosylated luminal domain, a single transmembrane segment, and a short cytoplasmic tail. We show that in highly purified lysosomes from rat liver, Lamp II, immunodetected with a monoclonal antibody on Western blots, it surprisingly distributed between a membrane bound form and a "soluble" form. The partition of the protein between the membrane and the content of lysosomes is strongly pH dependent. The soluble Lamp II population is sensitive to pH dependent aggregation as it is for many lysosomal content enzymes.
溶酶体相关膜蛋白II（Lamp II）是一种整合型I类糖蛋白。它由一个非常大且高度糖基化的腔结构域、一个单一的跨膜片段和一个短的细胞质尾巴组成。我们发现，在大鼠肝脏高度纯化的溶酶体中，用单克隆抗体在蛋白质印迹上进行免疫检测时，Lamp II令人惊讶地分布在膜结合形式和“可溶性”形式之间。该蛋白在溶酶体膜和内容物之间的分配强烈依赖于pH值。可溶性Lamp II群体对pH值依赖性聚集敏感，就像许多溶酶体内容物酶一样。
