Waldrop S M, Alexander D Z, Lowry R, Winn K J, Pearson T C, Constantinidis I
Department of Radiology, Frederik Philips Magnetic Resonance Research Center, Emory University School of Medicine, Atlanta, GA 30322, USA.
Biochem Biophys Res Commun. 1996 Jun 14;223(2):379-83. doi: 10.1006/bbrc.1996.0902.
This study defines the total sodium-23 magnetic resonance spectroscopy (23Na MRS) signal from in vivo heterotopic rat heart transplants in the early post-transplant period and examines the utility of this noninvasive method for monitoring allograft rejection. Measurements were performed at 4.7 T. Syngeneic (n = 6) and allogeneic (n = 6) donor hearts were transplanted into the neck of recipient rats. There were 27 MRS observations between days 0 and 29 post-transplant. Heart grafts were excised at various intervals post-transplant for histologic examination. Allogeneic heart grafts rejected between days 4 and 5 post-transplant while syngeneic grafts continued to beat. All hearts showed ischemic damage. Allogeneic hearts showed cellular rejection by Day 1. 23Na MRS showed a steady elevation in signal in the 3 days prior to rejection and a sharp rise after rejection. 23Na MRS accurately identified full rejection and was also sensitive to the rejection process.
本研究定义了移植后早期体内异位大鼠心脏移植的总钠-23磁共振波谱（23Na MRS）信号，并检验了这种非侵入性方法监测同种异体移植排斥反应的效用。测量在4.7 T条件下进行。将同基因（n = 6）和异基因（n = 6）供体心脏移植到受体大鼠颈部。在移植后0至29天之间进行了27次MRS观察。在移植后的不同时间间隔切除心脏移植物进行组织学检查。异基因心脏移植物在移植后第4至5天发生排斥，而同基因移植物继续跳动。所有心脏均显示缺血损伤。异基因心脏在第1天显示细胞排斥。23Na MRS显示在排斥前3天信号稳步升高，排斥后急剧上升。23Na MRS准确识别了完全排斥，并且对排斥过程也很敏感。
