Takashima Y, Takagi H, Takahashi M, Reinach P S, Mircheff A K, Warren D W, Yoshimura N
Department of Ophthalmology and Visual Science, Kyoto University Graduate School of Medicine, Kyoto 606, Japan.
Curr Eye Res. 1996 Jul;15(7):768-73. doi: 10.3109/02713689609003461.
Endothelin-1 (ET-1) has been found to accelerate rabbit corneal epithelial wound healing in vivo and proliferation of rabbit and bovine epithelial cells in vitro. We have now determined that endothelin-like immunoreactivity (ET-LI) is present in the tear fluid of New Zealand white rabbits, and we have addressed the question of whether it is produced by the tear glands (lacrimal gland and Harderian gland).
Tears were obtained with capillary pipettes and lacrimal gland fluid was collected by cannulation. ET-LI was determined with a sandwich-enzyme immunoassay. The reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to determine if there are gene transcripts for prepro ET-1 in tear glands. ET-LI in the tear glands was evaluated using immunohistochemistry.
The tears contained 13.85 +/- 3.94 pg/ml (mean +/- SEM, n = 6) ET-LI and the lacrimal gland fluid had 24.90 +/- 6.00 pg/ml (n = 6). The plasma contained 0.89 +/- 0.20 pg/ml (n = 6). Gene transcripts were identified for prepro ET-1 in the lacrimal and Harderian glands. Specific staining for ET-LI was found in the epithelial cells of the ducts of the lacrimal gland and the acinar cells of the Harderian gland.
The higher concentrations of ET-LI in the tears and the lacrimal gland fluid than in plasma along with prepro ET-1 gene transcripts in the tear glands suggest that they secrete ET-1.
已发现内皮素 -1（ET -1）可在体内加速兔角膜上皮伤口愈合，并在体外促进兔和牛上皮细胞增殖。我们现已确定新西兰白兔泪液中存在内皮素样免疫反应性（ET -LI），并探讨了其是否由泪腺（泪腺和哈德氏腺）产生的问题。
用毛细吸管获取眼泪，通过插管收集泪腺液。采用夹心酶免疫测定法测定ET -LI。运用逆转录聚合酶链反应（RT -PCR）来确定泪腺中是否存在前体ET -1的基因转录本。使用免疫组织化学评估泪腺中的ET -LI。
眼泪中ET -LI含量为13.85±3.94 pg/ml（平均值±标准误，n = 6），泪腺液中为24.90±6.00 pg/ml（n = 6）。血浆中为0.89±0.20 pg/ml（n = 6）。在泪腺和哈德氏腺中鉴定出前体ET -1的基因转录本。在泪腺导管的上皮细胞和哈德氏腺的腺泡细胞中发现了ET -LI的特异性染色。
眼泪和泪腺液中ET -LI浓度高于血浆，以及泪腺中存在前体ET -1基因转录本，表明它们分泌ET -1。
