钙离子在中国仓鼠卵巢细胞中触发大量胞吐作用。
Ca2+ triggers massive exocytosis in Chinese hamster ovary cells.
作者信息
Coorssen J R, Schmitt H, Almers W
机构信息
Abteilung Molekulare Zellforschung, Max-Planck-Institut fur Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany.
出版信息
EMBO J. 1996 Aug 1;15(15):3787-91.
Abstract
We have tracked the cell surface area of CHO cells by measuring the membrane capacitance, Cm. An increase in cytosolic [Ca2+], [Ca2+]i, increased the cell surface area by 20-30%. At micromolar [Ca2+]i the increase occurred in minutes, while at 20 microM or higher [Ca2+]i it occurred in seconds and was transient. GTPgammaS caused a 3% increase even at 0.1 microM [Ca2+]i. We conclude that CHO cells, previously thought capable only of constitutive exocytosis, can perform Ca2+-triggered exocytosis that is both massive and rapid. Ca2+-triggered exocytosis was also observed in 3T3 fibroblasts. Our findings add evidence to the view that Ca induces exocytosis in cells other than known secretory cells.
摘要
我们通过测量膜电容（Cm）来追踪中国仓鼠卵巢细胞（CHO细胞）的细胞表面积。胞质钙离子浓度（[Ca2+]i）的增加使细胞表面积增大了20% - 30%。在微摩尔浓度的[Ca2+]i下，这种增加在数分钟内发生，而在20微摩尔或更高浓度的[Ca2+]i时，增加在数秒内发生且是短暂的。即使在0.1微摩尔浓度的[Ca2+]i下，鸟苷三磷酸γ-硫酯（GTPγS）也会使细胞表面积增加3%。我们得出结论，之前认为仅能进行组成型胞吐作用的CHO细胞，能够进行大量且快速的钙离子触发的胞吐作用。在3T3成纤维细胞中也观察到了钙离子触发的胞吐作用。我们的发现为钙离子在已知分泌细胞以外的细胞中诱导胞吐作用这一观点增添了证据。
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