卵母细胞捐赠计划：严重男性因素不育或既往受精失败病例中卵胞浆内单精子注射的结果

Oocyte donation programme: results obtained with intracytoplasmic sperm injection in cases of severe male factor infertility or previous failed fertilization.

作者信息

Borini A, Bafaro M G, Bianchi L, Violini F, Bonu M A, Flamigni C

机构信息

TECNOBIOS, Centre for Reproductive Health, Bologna, Italy.

出版信息

Hum Reprod. 1996 Mar;11(3):548-50. doi: 10.1093/humrep/11.3.548.

DOI:10.1093/humrep/11.3.548

PMID:8671263

Abstract

Intracytoplasmic sperm injection was carried out in 15 oocyte donation cycles of 15 infertile couples where oocytes had failed to fertilize after in-vitro fertilization (IVF) procedures or where the male partner had severe male factor infertility. A total of 62 oocytes were donated, but only 46 of these, in metaphase II, were injected. Of the injected oocytes, 31 (67.3%) had two pronuclei the morning after the injection procedure. On the following day, 29 embryos were obtained (93% of the fertilized oocytes) and 25 were transferred. Two patients were not successful and consequently did not undergo embryo transfer. A total of five clinical pregnancies were obtained, giving pregnancy rates of 33.3 and 38.4% per started cycle and embryo transfer respectively.

摘要

对15对不育夫妇进行了15个卵母细胞捐赠周期的胞浆内单精子注射，这些夫妇的卵母细胞在体外受精（IVF）程序后未能受精，或者男性伴侣患有严重的男性因素不孕症。总共捐赠了62个卵母细胞，但其中只有46个处于中期II的卵母细胞接受了注射。在注射后的早晨，31个（67.3%）注射的卵母细胞出现了两个原核。第二天，获得了29个胚胎（占受精卵母细胞的93%），并移植了25个胚胎。两名患者未成功，因此未进行胚胎移植。总共获得了5次临床妊娠，起始周期和胚胎移植的妊娠率分别为33.3%和38.4%。