Paul M, Sumpter J P, Lindsay K S
Fertility Laboratory, Hammersmith Hospitals Trust, Queen Charlotte's and Chelsea Hospital, London, UK.
Hum Reprod. 1996 Apr;11(4):814-9. doi: 10.1093/oxfordjournals.humrep.a019259.
In the presence of pentoxifylline, human spermatozoa are induced to increase certain motion characteristics; however, the role of this drug in fertilization remains equivocal. In this study, the influence of pentoxifylline on one aspect of fertilization, that is sperm-zona binding, has been examined. Results from a fluorescence label competitive zona binding (CZB) test showed that spermatozoa exposed to a pentoxifylline challenge of between 0.1 and 5 mM, which was curtailed after 1 h by washing, had a decreased (P < 0.01) ability to bind to intact zona compared with control spermatozoa. The washing procedures also removed (decrease P < 0.01 compared with peak values) some of the enhanced motion characteristics induced by pentoxifylline. These results were in contrast with those obtained using experimental conditions that maintained an increased curvilinear velocity (VCL) and lateral head displacement (ALH) (increase P < 0.001 above baseline controls) in the continued presence of pentoxifylline. Using a hemizona binding (HZB) assay, 3 mM pentoxifylline increased (P < 0.001) sperm-zona binding almost 20% above zona binding with unexposed control spermatozoa. It was concluded that, in the presence of pentoxifylline, there is increased sperm binding to the zona pellucida; however, if the drug is removed by washing, the sperm binding to the zona is decreased in concert with the removal of the enhanced motion characteristics. The application of zona solubilization by acidic conditions in a microchamber enabled the precise determination of sperm numbers in both of the sperm-zona binding assays, and the results demonstrated that a wide variation in sperm numbers was observed in each test, with 63-580 spermatozoa bound in the CZB assay and 56-1340 spermatozoa bound on a hemizona.
在己酮可可碱存在的情况下，人类精子会被诱导增加某些运动特征；然而，这种药物在受精过程中的作用仍不明确。在本研究中，已对己酮可可碱对受精的一个方面，即精子与透明带结合的影响进行了检测。荧光标记竞争透明带结合（CZB）试验结果显示，暴露于0.1至5 mM己酮可可碱挑战下1小时后经洗涤处理的精子，与对照精子相比，其与完整透明带结合的能力降低（P < 0.01）。洗涤过程还消除了（与峰值相比P < 0.01）己酮可可碱诱导的一些增强的运动特征。这些结果与在己酮可可碱持续存在的情况下保持曲线速度（VCL）和头部横向位移（ALH）增加（比基线对照增加P < 0.001）的实验条件下获得的结果形成对比。使用半透明带结合（HZB）试验，3 mM己酮可可碱使精子与透明带的结合增加（P < 0.），比未暴露的对照精子与透明带的结合高出近20%。得出的结论是，在己酮可可碱存在的情况下，精子与透明带的结合增加；然而，如果通过洗涤去除该药物，精子与透明带的结合会随着增强的运动特征的去除而降低。在微室中通过酸性条件使透明带溶解，能够精确测定两种精子与透明带结合试验中的精子数量，结果表明在每次试验中观察到精子数量有很大差异，在CZB试验中有63 - 580个精子结合，在半透明带上有56 - 1340个精子结合。
