Arakawa H, Shimizu T, Takeda S
Basel Institute for Immunology, Grenzacherstrasse 487, 4005 Basel, Switzerland.
Int Immunol. 1996 Jan;8(1):91-9. doi: 10.1093/intimm/8.1.91.
V-J arrangements at Ig light chain (IgL) genes occur in resting small pre-B cells. In the absence of cell division, the probability of production kappa and lambda rearrangements is proportional to the output of kappa+ B and lambda+ B cells in bone marrow. The kinetics and probability of productive kappa or lambda rearrangements was assessed in three groups of mice carrying two (wild-type), one or no intact Igkappa gene, and the following conclusion are drawn. Kappa and lambda rearrangements occur independently at different kinetics, and rearrangements are initiated at a time when kappa rearrangements are stopping. The probability of productive kappa and lambda rearrangements per chromosome is calculated to be approximately 60 and approximately 20% respectively. Thus, a kappa gene can attempt rearrangements up to three times per chromosome during B cell development. These findings explain that the observed ratio of kappa+ B/lambda+ B cell production in wild-type mice is 95/5.
免疫球蛋白轻链（IgL）基因的V-J重排在静止的小前B细胞中发生。在无细胞分裂的情况下，κ和λ重排产生的概率与骨髓中κ+B细胞和λ+B细胞的产出成正比。在三组分别携带两个（野生型）、一个或没有完整Igκ基因的小鼠中评估了有功能的κ或λ重排的动力学和概率，并得出以下结论。κ和λ重排在不同的动力学过程中独立发生，且重排在κ重排停止时启动。每条染色体上有功能的κ和λ重排的概率分别计算为约60%和约20%。因此，在B细胞发育过程中，一个κ基因每条染色体最多可尝试三次重排。这些发现解释了在野生型小鼠中观察到的κ+B/λ+B细胞产生比例为95/5的现象。
