Rainaldi G, Meneveri R, Mariani L, Ginelli E, Moretti A, Vatteroni L
Genetica e Biochimica Tossicologica, Istituto di Mutagenesi e Differenziamento, CNR, Via Svezia 10, Pisa, Italy.
Mutagenesis. 1996 Jul;11(4):401-4. doi: 10.1093/mutage/11.4.401.
Clone CSA7 is a CHEF18 hamster cell line that shows an increased intracellular accumulation of dCTP. To localize the mutations that accumulate spontaneously in a functional gene of such a mutator phenotype, independent CSA7 mutants of the hypoxanthine-guanine phosphoribosyl transferase (hprt) gene were isolated and screened by a polymerase chain reaction-single strand conformation polymorphism technique. Sixty-two percent of mutants produced detectable changes of the strand migration profile and the mutations were preferentially localized in the exons 3 (31%) and 6 (62%). The sequencing of such exons revealed that the rate of C base incorporation was the major mutation pathway and that the A base of a GGA sequence was the preferential site of misincorporation.
克隆株CSA7是一种CHEF18仓鼠细胞系，其细胞内dCTP积累增加。为了定位在具有这种突变表型的功能基因中自发积累的突变，分离了次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶（hprt）基因的独立CSA7突变体，并通过聚合酶链反应 - 单链构象多态性技术进行筛选。62%的突变体产生了可检测到的链迁移图谱变化，且突变优先定位在外显子3（31%）和外显子6（62%）中。对外显子进行测序发现，C碱基掺入率是主要的突变途径，并且GGA序列中的A碱基是错掺入的优先位点。
