雀鳝（Lepisosteus osseus）生长激素cDNA的克隆：辐鳍鱼纲生长激素结构的趋势

Cloning of a gar (Lepisosteus osseus) GH cDNA: trends in actinopterygian GH structure.

作者信息

Rubin D A, Youson J H, Marra L E, Dores R M

机构信息

Department of Biological Sciences, University of Denver, Colorado 80208, USA.

出版信息

J Mol Endocrinol. 1996 Feb;16(1):73-80. doi: 10.1677/jme.0.0160073.

DOI:10.1677/jme.0.0160073

PMID:8672235

Abstract

A cDNA containing the sequence of GH was cloned and sequenced from a pituitary cDNA library for the holostean fish Lepisosteus osseus (common name: gar). The gar GH cDNA contained an open reading frame of 633 nucleotides and a 3' untranslated region (including the terminal codon TAG) of 1058 nucleotides. The overall length of the gar GH cDNA including leader sequence, signal sequence, hormone sequence and 3' untranslated region was 1713 nucleotides. Thus, the gar GH cDNA is the largest vertebrate GH cDNA yet cloned. A comparison of GH sequences from ancient (holostean fishes-gar and bowfin; one chondrostean fish-the Russian sturgeon) and more modern (27 species of teleosts) members of class Actinopterygii indicate that members of this class have maintained many of the invariant residues deemed necessary for GH folding motifs (intramolecular relationships) observed in mammals.

摘要

从全骨鱼类硬骨舌鱼（俗称雀鳝）的垂体cDNA文库中克隆并测序了一个包含生长激素（GH）序列的cDNA。雀鳝生长激素cDNA包含一个633个核苷酸的开放阅读框和一个1058个核苷酸的3'非翻译区（包括终止密码子TAG）。包括前导序列、信号序列、激素序列和3'非翻译区在内的雀鳝生长激素cDNA的全长为1713个核苷酸。因此，雀鳝生长激素cDNA是迄今克隆的最大的脊椎动物生长激素cDNA。对古代（全骨鱼类——雀鳝和弓鳍鱼；一种软骨硬鳞鱼类——俄罗斯鲟鱼）和更现代的（27种硬骨鱼类）辐鳍鱼纲成员的生长激素序列进行比较表明，该纲成员保留了许多在哺乳动物中观察到的、被认为对生长激素折叠基序（分子内关系）必不可少的不变残基。