通过靶向神经氨酸酶编码基因的聚合酶链反应检测脆弱拟杆菌。

Detection of Bacteroides fragilis by PCR assay targeting the neuraminidase-encoding gene.

作者信息

Kuwahara T, Akimoto S, Ugai H, Kamogashira T, Kinouchi T, Ohnishi Y

机构信息

Department of Bacteriology, School of Medicine, University of Tokushima, Japan.

出版信息

Lett Appl Microbiol. 1996 May;22(5):361-5. doi: 10.1111/j.1472-765x.1996.tb01179.x.

DOI:10.1111/j.1472-765x.1996.tb01179.x

PMID:8672275

Abstract

Oligonucleotide primers were designed on the basis of the sequence of the neuraminidase-encoding gene (nanH) of Bacteroides fragilis and used for the specific detection of this anaerobe by the nested PCR assay. Fifty-nine of 60 representative strains of Bact. fragilis were detected, while none of 45 strains of other species generated visible PCR products. The detection limits of Bact. fragilis cells and DNA by the nested PCR were 10 colony-forming units and 10 fg of chromosomal DNA, respectively. The PCR assay targeting the nanH gene has the potential for the detection of Bact. fragilis.

摘要

根据脆弱拟杆菌神经氨酸酶编码基因（nanH）的序列设计了寡核苷酸引物，并用于通过巢式PCR检测法对这种厌氧菌进行特异性检测。在60株脆弱拟杆菌代表性菌株中，检测到了59株，而其他45个菌种的菌株均未产生可见的PCR产物。巢式PCR对脆弱拟杆菌细胞和DNA的检测限分别为10个菌落形成单位和10 fg染色体DNA。针对nanH基因的PCR检测法具有检测脆弱拟杆菌的潜力。