Ono T, Akimoto S, Kinouchi T, Kataoka K, Ohnishi Y
Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
FEMS Microbiol Lett. 1994 Aug 15;121(2):153-8. doi: 10.1111/j.1574-6968.1994.tb07092.x.
A neuraminidase-encoding gene nanH of Bacteroides fragilis strain YCH46 was cloned into the cosmid vector pHC79. The nanH gene was subcloned from the cosmid and was located within a 2.2-kb XhoI-KpnI fragment. Southern hybridization experiments demonstrated that the gene was present as a single copy on the bacterial chromosome. Neuraminidase activity expressed in the initial Escherichia coli clone was approximately 3600-fold lower than that expressed in B. fragilis YCH46. However, when nanH was transferred from E. coli to B. uniformis by mobilization of a shuttle plasmid, the transconjugant expressed 1100-fold higher activity than the E. coli donor did. These results suggest that modes of nanH expression in E. coli and Bacteroides are heterologous.
