Astete S G, Leigh J A
Department of Microbiology, University of Washington, Seattle, 98195, USA.
Mol Plant Microbe Interact. 1996 Jul;9(5):395-400. doi: 10.1094/mpmi-9-0395.
In addition to the exopolysaccharide succinoglycan, Rhizobium meliloti can produce a galactoglucan exopolysaccharide, EPS II. The production of EPS II occurs in certain mutant strains, in strains containing extra copies of EPS II synthesis genes, or in the wild-type strain under phosphate-limiting conditions. We have identified a gene, mucS, that is in a locus required for EPS II induction by extra gene copies and by phosphate limitation, and that activates the expression of at least one other EPS II synthesis gene. mucS lies within a cluster of EPS II synthesis genes and contains an open reading frame of 190 amino acids. MucS does not show any significant similarity to known genes and may represent a new type of regulatory protein.
除了胞外多糖琥珀聚糖外，苜蓿根瘤菌还能产生一种半乳葡聚糖胞外多糖，即EPS II。EPS II的产生发生在某些突变菌株中、含有EPS II合成基因额外拷贝的菌株中，或在磷酸盐限制条件下的野生型菌株中。我们已经鉴定出一个基因，mucS，它位于由额外基因拷贝和磷酸盐限制诱导EPS II所需的位点，并且激活至少一个其他EPS II合成基因的表达。mucS位于EPS II合成基因簇内，包含一个190个氨基酸的开放阅读框。MucS与已知基因没有任何显著的相似性，可能代表一种新型的调节蛋白。
