葡萄病原菌根癌土壤杆菌中酒石酸盐利用基因的特征与分布

Characterization and distribution of tartrate utilization genes in the grapevine pathogen Agrobacterium vitis.

作者信息

Salomone J Y, Crouzet P, De Ruffray P, Otten L

机构信息

Department of Phytopathology, Plant Molecular Biology Institute of the C.N.R.S., Strasbourg, France.

出版信息

Mol Plant Microbe Interact. 1996 Jul;9(5):401-8. doi: 10.1094/mpmi-9-0401.

DOI:10.1094/mpmi-9-0401

PMID:8672817

Abstract

Agrobacterium vitis is a common pathogen of grapevine. Most strains utilize tartrate, an abundant compound in grapevine. Strain AB3 carries two tartrate utilization (or TAR) regions: TAR-I (on the large pTrAB3 plasmid) and TAR-II (on the AB3 Ti plasmid). TAR-I and TAR-II were structurally and functionally analyzed and are similar to the TAR-III region from the tartrate utilization plasmid pTrAB4 of the nopaline-type A. vitis strain AB4 (Crouzet and Otten, J. Bacteriol. 1995, 177:6518-6526). The minimal tartrate utilization region of TAR-I contains four genes (ttuA-ttuD). The ttuC gene is homologous to the tartrate dehydrogenase gene from Pseudomonas putida. Outside the minimal region a second ttuC-like gene is found (ttuC') which is transcribed and complements a ttuC mutant. Most grapevine isolates carry one or two of the three characterized TAR regions and show a considerable degree of polymorphism around these regions.

摘要

葡萄土壤杆菌是葡萄的常见病原体。大多数菌株利用酒石酸盐，这是葡萄中一种丰富的化合物。AB3菌株携带两个酒石酸盐利用（或TAR）区域：TAR-I（位于大的pTrAB3质粒上）和TAR-II（位于AB3 Ti质粒上）。对TAR-I和TAR-II进行了结构和功能分析，它们与胭脂碱型葡萄土壤杆菌菌株AB4的酒石酸盐利用质粒pTrAB4中的TAR-III区域相似（Crouzet和Otten，《细菌学杂志》1995年，177:6518-6526）。TAR-I的最小酒石酸盐利用区域包含四个基因（ttuA-ttuD）。ttuC基因与恶臭假单胞菌的酒石酸脱氢酶基因同源。在最小区域之外发现了第二个类似ttuC的基因（ttuC'），它被转录并能互补ttuC突变体。大多数葡萄分离株携带三个已鉴定的TAR区域中的一个或两个，并且在这些区域周围表现出相当程度的多态性。