Bouma G J, Schanz U, Oudshoorn M, van der Meer-Prins P M, Roelen D L, van Rood J J, Claas F H
Department of Immunohematology and Bloodbank, Leiden University Hospital, Netherlands.
Bone Marrow Transplant. 1996 Jan;17(1):19-23.
We have developed a limiting dilution analysis-assay which makes the determination of both helper and cytotoxic T lymphocyte precursor frequencies possible. The way in which T helper precursor frequencies are determined in this combined assay is essentially the same as the method published previously. Experimental conditions for the combined assay have been optimized to obtain cytotoxic T lymphocyte precursor frequencies that are comparable to those determined in the assay routinely used in our laboratory. For all situations where the amount of available cell material is a limiting factor, it will be an advantage that with the same number of cells needed for a standard cytotoxic T lymphocyte precursor frequency determination, in the combined assay both helper and cytotoxic T lymphocyte precursor frequencies can be determined. This is especially convenient in the case of bone marrow transplantation, where evidence is accumulating that low or negative cytotoxic T lymphocyte and/or T helper precursor frequencies may be a prerequisite for a successful transplantation with a minimal risk of graft-versus-host disease.
我们开发了一种极限稀释分析检测方法，该方法能够同时测定辅助性T淋巴细胞和细胞毒性T淋巴细胞前体频率。在这种联合检测中，测定辅助性T淋巴细胞前体频率的方法与之前发表的方法基本相同。联合检测的实验条件已得到优化，以获得与我们实验室常规使用的检测方法所测定的细胞毒性T淋巴细胞前体频率相当的结果。对于所有可用细胞材料量为限制因素的情况，在联合检测中，使用与标准细胞毒性T淋巴细胞前体频率测定所需相同数量的细胞，就能够同时测定辅助性T淋巴细胞和细胞毒性T淋巴细胞前体频率，这将是一个优势。在骨髓移植的情况下，这一点尤其方便，因为越来越多的证据表明，低或阴性的细胞毒性T淋巴细胞和/或辅助性T淋巴细胞前体频率可能是以最小移植物抗宿主病风险成功进行移植的先决条件。
