Wackym P A, Chen C T, Ishiyama A, Pettis R M, López I A, Hoffman L
Department of Otolaryngology, Mount Sinai School of Medicine, New York, NY 10029-6574, USA.
Cell Biol Int. 1996 Mar;20(3):187-92. doi: 10.1006/cbir.1996.0023.
The expression of the five muscarinic acetylcholine receptor (mAChR) subtypes (m1-m5) in the vestibular end-organs and in the primary afferent vestibular ganglia of the human and rat was studied using RT-PCR from the two tissue populations from both species. In the human, although all five mAChR subtypes were expressed in brain, only the m1, m2, and m5 mAChR subtypes were amplified from both the vestibular ganglia and the vestibular end-organs, while in the rat, all five mAChR subtypes were expressed. These data suggest that the efferent cholinergic axo-dendritic and axo-somatic synapses have a muscarinic component and that there are pharmacologic implications for patients with vestibular dysfunction.
利用逆转录聚合酶链反应（RT-PCR）技术，对人和大鼠的前庭终器及前庭初级传入神经节中五种毒蕈碱型乙酰胆碱受体（mAChR）亚型（m1 - m5）的表达进行了研究，所用组织样本来自这两个物种的上述两种组织。在人类中，尽管所有五种mAChR亚型在脑中均有表达，但仅在前庭神经节和前庭终器中扩增出了m1、m2和m5 mAChR亚型；而在大鼠中，所有五种mAChR亚型均有表达。这些数据表明，传出胆碱能轴-树突突触和轴-体突触具有毒蕈碱成分，并且对前庭功能障碍患者具有药理学意义。
