Nagnan-Le Meillour P, Huet J C, Maibeche M, Pernollet J C, Descoins C
Unité de Phytopharmacie et des Médiateurs Chimiques, INRA, Versailles, France.
Insect Biochem Mol Biol. 1996 Jan;26(1):59-67. doi: 10.1016/0965-1748(95)00062-3.
Proteins extracted form the antennae of Mamestra brassicae (L.) (Lepidoptera: Noctuidae) adults were biochemically characterized as pheromone-binding proteins (PBP) and general odorant-binding proteins (GOBP). PBP and GOBP were purified by two successive and different HPLC (high performance liquid chromatography) systems and native polyacrylamide gel electrophoresis (native-PAGE). Their N-terminal sequence was determined by Edman microsequencing. The combined results showed evidence for three different PBPs in males, and two different PBPs in females. In addition, one GOBP was characterized in both males than in females antennae. In the males, two isoforms of PBP have the same N-terminal sequence, but different apparent mobilities and hydrophobicities: they could be separated by electrophoresis and reverse phase-HPLC (RP-HPLC). The other PBP sequence (SQEIM) showed particularly high homology (88%) with the PBP of Heliothis virescens, another noctuid moth. The existence of several forms of PBP in the same animal strongly supports the hypothesis of the specificity of binding between the proteins and their odorant ligands, the pheromonal compounds. The observed microdiversity at the soluble proteins level could provide a good model for studying their involvement in the initial stages of odor discrimination.
从甘蓝夜蛾成虫触角中提取的蛋白质经生化鉴定为性信息素结合蛋白（PBP）和普通气味结合蛋白（GOBP）。通过两个连续且不同的高效液相色谱（HPLC）系统和天然聚丙烯酰胺凝胶电泳（native-PAGE）对PBP和GOBP进行了纯化。通过埃德曼微量测序法测定了它们的N端序列。综合结果表明，雄性中有三种不同的PBP，雌性中有两种不同的PBP。此外，在雄性触角中鉴定出一种GOBP，其含量高于雌性。在雄性中，两种PBP同工型具有相同的N端序列，但表观迁移率和疏水性不同：它们可通过电泳和反相高效液相色谱（RP-HPLC）分离。另一种PBP序列（SQEIM）与另一种夜蛾绿铃夜蛾的PBP具有特别高的同源性（88%）。同一动物体内存在多种形式的PBP，有力地支持了蛋白质与其气味配体（即性信息素化合物）之间结合特异性的假说。在可溶性蛋白质水平上观察到的微观多样性可为研究它们在气味辨别初始阶段的作用提供一个良好的模型。
