Nadon N L, Draeger K
Department of Biological Sciences, University of Tulsa, OK 74104, USA.
Transgenic Res. 1996 May;5(3):209-11. doi: 10.1007/BF01969711.
The protocol described in this report provides a simple, accurate and efficient assay for detection of transgenes and mutations in large colonies of rodents, using crude lysates prepared from the digit cut from the animals for identification purposes. This can be done as early as 6 days of age, minimizing trauma to the mice and allowing assays to be completed long before weaning.
本报告中描述的方案提供了一种简单、准确且高效的检测方法，用于在大型啮齿动物群体中检测转基因和突变，该方法使用从动物身上切下的趾头制备的粗裂解物进行鉴定。这最早可在6日龄时进行，将对小鼠的创伤降至最低，并使检测在断奶前很久就能完成。
