Gajkowska B, Gadamski R, Mossakowski M
Laboratory of Ultrastructure of Central Nervous System, Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1995;33(1):5-19.
The subcellular distribution of glutamate and GABA in synapses of Mongolian gerbils hippocampus was examined using post-embedding immunogold staining method for electron microscopy. Immunolabelling was performed with 10 nm gold-antibody complexes for glutamate and GABA. The gold particle densities gave reliable information about the relative concentrations of these amino acid neurotransmitters. Our results indicate that ischemia leads to the temporal decrease of GABA-like immunoreactivity in symmetric synapses and a slight enhancement of the level of glutamate-like reactivity in asymmetric ones. The striking finding was a redistribution of glutamate-like immunoreactivity from neurons to glia. This suggests the capacity of glia to metabolize the excess of glutamate after ischemia. The disturbances at the level of neurotransmitters and their possible role in hippocampal neuronal injury were stressed.
采用包埋后免疫金染色电镜技术，研究了蒙古沙鼠海马突触中谷氨酸和γ-氨基丁酸（GABA）的亚细胞分布。用针对谷氨酸和GABA的10nm金标抗体复合物进行免疫标记。金颗粒密度提供了有关这些氨基酸神经递质相对浓度的可靠信息。我们的结果表明，缺血导致对称突触中GABA样免疫反应性暂时降低，不对称突触中谷氨酸样反应性水平略有升高。显著的发现是谷氨酸样免疫反应性从神经元重新分布到神经胶质细胞。这表明神经胶质细胞在缺血后具有代谢过量谷氨酸的能力。强调了神经递质水平的紊乱及其在海马神经元损伤中可能的作用。
