Kirkpatrick J S, Seaber A V, Glisson R R, Bassett F H
Division of Orthopaedic Surgery, Duke University Medical Center, Durham, NC, USA.
J South Orthop Assoc. 1996 Spring;5(1):20-9.
This study was done to determine the clinical and biomechanical properties of cryopreserved anterior cruciate ligament allografts at 3, 6, 9, and 24 months after transplantation. A companion study of autografts was done to evaluate the effects of the surgical procedure and preservation on the tissue. The knee joints of 69 mongrel dogs (allograft n = 34, autograft n = 35) weighing 17 kg to 25 kg were grafted. Biomechanical results showed that maximum load was less in allografts than in autografts at each time interval. Deformation and slope were not significantly different between allograft and autograft. An apparent delay in revascularization and cellular repopulation was found in allografts compared with autografts, and collagen type I/type III ratios were similar in both grafts. DNA analysis indicated complete replacement of DNA in the graft by the host. Allografts provide adequate functional stability for daily activities in the canine model up to 2 years after transplantation. Biomechanical properties were relatively poor in allografts, which may lead to failure under more strenuous activity. The biologic similarity to autografts may indicate future remodeling potential.
本研究旨在确定冷冻保存的前交叉韧带同种异体移植物在移植后3个月、6个月、9个月和24个月时的临床和生物力学特性。同时进行了一项自体移植物的对照研究，以评估手术操作和保存对组织的影响。对69只体重在17千克至25千克之间的杂种犬（同种异体移植组n = 34，自体移植组n = 35）的膝关节进行了移植。生物力学结果显示，在每个时间间隔，同种异体移植物的最大负荷均低于自体移植物。同种异体移植物和自体移植物之间的变形和斜率无显著差异。与自体移植物相比，同种异体移植物中血管再生和细胞重新填充明显延迟，且两种移植物中的I型/III型胶原比率相似。DNA分析表明移植物中的DNA已被宿主完全替代。在犬模型中，同种异体移植物在移植后长达2年的时间里可为日常活动提供足够的功能稳定性。同种异体移植物的生物力学特性相对较差，在更剧烈的活动下可能导致失败。与自体移植物的生物学相似性可能表明其未来具有重塑潜力。
