Nicolas J C, Boussioux A M, Joyeux A, Balaguer P, Térouanne B, Roux S, Gagne D, Pons M
Centre de Recherches INSERM, Montpellier, France.
C R Seances Soc Biol Fil. 1995;189(5):740-53.
Bioluminescence and chemiluminescence have emerged in the last decade as a major tool for biochemical and biological studies. Several very sensitive assays have been developed in our laboratory such as enzymatic assays, immunoassays and detection of nucleic acids. The use of a new instrumentation allowing to dimensional photon counting has permit the emergence of new investigations at the cellular level. In addition to the advantages of sensitivity and the real-time non-invasive nature of this detection system, the imaging potential of using low-light and photon counting video cameras has been particularly influential in establishing its ascendence over more traditional systems. This review provides a reflection in this field through several applications in life sciences.
在过去十年中，生物发光和化学发光已成为生物化学和生物学研究的主要工具。我们实验室已经开发了几种非常灵敏的检测方法，如酶促检测、免疫检测和核酸检测。一种允许进行三维光子计数的新型仪器的使用，使得细胞水平的新研究得以出现。除了这种检测系统具有灵敏度高和实时非侵入性的优点外，使用低光和光子计数摄像机的成像潜力在确立其相对于更传统系统的优势方面尤其具有影响力。本综述通过生命科学中的几个应用对该领域进行了思考。
