子宫过氧化物酶对雌二醇的代谢：水溶性产物的性质

Metabolism of estradiol by uterine peroxidase: nature of the water-soluble products.

作者信息

Jellinck P H, McNabb T

出版信息

Steroids. 1977 Apr;29(4):525-37. doi: 10.1016/0039-128x(77)90072-1.

Abstract

The nature of the water-soluble products formed by incubating labelled estradiol with uterine peroxidase in the presence of H2O2 and tyrosine was examined by two-dimensional thin-layer chromatography and high voltage electrophoresis. It was shown that the steroid and amino acid were associated in a 1:2 or 1:3 ratio and evidence was provided by 3H-exchange for the interaction of tyrosine with ring A of estradiol at C-2 and C-4. The possible role of estrogen-induced peroxidase in the uterus in vivo is discussed.

摘要

通过二维薄层色谱法和高压电泳法，对在过氧化氢和酪氨酸存在的情况下，将标记的雌二醇与子宫过氧化物酶一起孵育所形成的水溶性产物的性质进行了研究。结果表明，类固醇与氨基酸以1:2或1:3的比例结合，并且通过³H交换证明了酪氨酸在C-2和C-4处与雌二醇的A环相互作用。文中还讨论了雌激素诱导的过氧化物酶在体内子宫中的可能作用。