Sato H, Borchman D, Ozaki Y, Lamba O P, Byrdwell W C, Yappert M C, Paterson C A
Department of Ophthalmogy and Visual Sciences, University of Louisville, KY 40292, USA.
Exp Eye Res. 1996 Jan;62(1):47-53. doi: 10.1006/exer.1996.0006.
In other systems, proteins have been shown to alter the molecular structures of lipids in the cell membrane bilayer. We wished to determine if proteins altered the structure of lens lipids. The structure of lipid hydrocarbon chains in urea purified human lens membrane vesicles containing intrinsic, hydrophobically bound proteins was compared to the structure of lipids in vesicles without protein. Fourier transform Raman spectroscopy was used to characterize lipid and protein structure. To study lipid interactions with extrinsic, surface bound proteins, the lipid structure was compared in bovine lipid vesicles with and without alpha-crystallin bound to the surface of the membrane. Lipid structure was studied using Fourier transform infrared spectroscopy. No change in lipid structure was detected even at protein/lipid weight ratios of two to one. Human lens intrinsic proteins contained a high amount of a helical structure (60%), but did not alter hydrocarbon chain interactions.
在其他系统中，已有研究表明蛋白质会改变细胞膜双层中脂质的分子结构。我们希望确定蛋白质是否会改变晶状体脂质的结构。将含有内在疏水结合蛋白的尿素纯化人晶状体膜囊泡中脂质烃链的结构与不含蛋白质的囊泡中脂质的结构进行比较。利用傅里叶变换拉曼光谱对脂质和蛋白质结构进行表征。为了研究脂质与外在表面结合蛋白的相互作用，比较了有和没有α-晶状体蛋白结合到膜表面的牛脂质囊泡中的脂质结构。使用傅里叶变换红外光谱研究脂质结构。即使在蛋白质/脂质重量比为2:1的情况下，也未检测到脂质结构的变化。人晶状体内在蛋白含有大量的α螺旋结构（60%），但不会改变烃链相互作用。
