Praillet C, Lortat-Jacob H, Grimaud J A
Laboratoire de Pathologie des Fibroses, CNRS URA 1459, Institut Pasteur de Lyon, France.
FEBS Lett. 1996 Jun 3;387(2-3):109-12. doi: 10.1016/0014-5793(96)00468-1.
Glycosaminoglycans synthesized by human skin fibroblasts were simultaneously radiolabelled with D-[1-(3H)]glucosamine and Na2(35)SO4. Considering 3H incorporation, we found that IFNgamma increased the production of glycosaminoglycan synthesis, including hyaluronic acid, heparan and chondroitin/dermatan sulfate. In contrast, the production of heparan and chondroitin/dermatan sulfate was slightly decreased on the basis of the 35S signal. Furthermore, when heparan sulfate was treated with nitrous acid, the release of free 35S was greater in control than in treated cells, although the 3H patterns of depolymerization with this agent were similar. These data demonstrate that IFNgamma inhibits the incorporation of sulfate from extracellular medium into heparan sulfate.
用人皮肤成纤维细胞合成的糖胺聚糖同时用D-[1-(3H)]葡糖胺和Na2(35)SO4进行放射性标记。考虑到3H的掺入，我们发现γ干扰素增加了包括透明质酸、硫酸乙酰肝素和硫酸软骨素/硫酸皮肤素在内的糖胺聚糖合成的产量。相比之下，基于35S信号，硫酸乙酰肝素和硫酸软骨素/硫酸皮肤素的产量略有下降。此外，当硫酸乙酰肝素用亚硝酸处理时，对照细胞中游离35S的释放量比处理过的细胞更多，尽管用该试剂解聚的3H模式相似。这些数据表明，γ干扰素抑制细胞外培养基中的硫酸盐掺入硫酸乙酰肝素。
