Shimooku K, Akisue T, Jinnai H, Hitomi T, Ogino C, Yoshida K, Nakamura S, Nishizuka Y
Department of Biochemistry, Kobe Univeristy School of Medicine, Kobe, Japan.
FEBS Lett. 1996 Jun 3;387(2-3):141-4. doi: 10.1016/0014-5793(96)00483-8.
For activation of kidney membrane phospholipase D (PLD), cytosol is absolutely needed in addition to GTP-gamma-S. The active component of cytosol consists of three protein factors: ADP-ribosylation factor, RhoA, and a soluble 36-kDa protein. Any combination of these two factors synergistically activates PLD to some extent, but the presence of the three factors causes full activation. The 36-kDa protein is stable at 60 degrees C but inactivated at 80 degrees C for 10 min. Tissue distribution of the 36-kDa protein roughly coincides with that of PLD, suggesting physiological relevance of the protein in the regulation of PLD.
