中间普氏菌和变黑普氏菌的血清型、核糖体型及结合特性

Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes and binding characteristics.

作者信息

Dahlén G G, Johnson J R, Gmür R

机构信息

Department of Oral Microbiology, Faculty of Odontology, University of Göteborg, Sweden.

出版信息

FEMS Microbiol Lett. 1996 Apr 15;138(1):89-95. doi: 10.1111/j.1574-6968.1996.tb08140.x.

DOI:10.1111/j.1574-6968.1996.tb08140.x

PMID:8674976

Abstract

Type strains and 62 clinical isolates of Prevotella intermedia and Prevotella nigrescens were typed with the use of genomic DNA fingerprints and rRNA gene probes. The strains were further serotyped with monoclonal antibodies and characterized with SDS-PAGE, enzymatic activities, hemolysis and hemagglutination and coaggregation with Streptococcus and Actinomyces spp. P. intermedia and P. nigrescens were found to have distinct ribotype patterns which correspond to previously defined serotyupes I and II/III, respectively. No clear phenotypic difference related to hemolysis, hemagglutination and coaggregation with streptococcus and actinomyces species, or expression of aminopeptides and lipase was found between P. intermedia and P. migrescens.

摘要

使用基因组DNA指纹图谱和rRNA基因探针，对中间普氏菌和变黑普氏菌的模式菌株及62株临床分离株进行分型。用单克隆抗体对这些菌株进一步进行血清分型，并用SDS - PAGE、酶活性、溶血、血凝以及与链球菌和放线菌属的共聚作用进行鉴定。发现中间普氏菌和变黑普氏菌具有不同的核糖体分型模式，分别对应于先前定义的血清型I和II/III。在中间普氏菌和变黑普氏菌之间，未发现与溶血、血凝、与链球菌和放线菌属的共聚作用或氨肽酶和脂肪酶表达相关的明显表型差异。